Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities

Fatin, R. J. and Wahab, Razak and Mohd. Daud, Jamaludin and Sudin, Mahmud and Mat Rasat, Mohd Sukhairi and Sulaiman, Othman (2012) Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities. Current Research Journal of Biological Science, 4 (5). pp. 600-607. ISSN 2041-076X E-ISSN 2041-0778

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Abstract

The present study investigated the Total Phenolic Content (TPC) and free radical scavenging activity of Nauclea subdita (Korth) Steud. which might reflect the potency of the plant extract as antioxidant. Young and matured trees of N. subdita were harvested and cut into three heartwood parts (top, middle and bottom) and grounded into powdered forms. Soxhlet extraction of the heartwood samples using polar (methanol) and non-polar (hexane) solvents and phytochemical screening tests were carried out. The total phenolic contents of the methanolic crude extracts from heartwood parts were determined by colorimetry at 760 nm using Folin-Ciocalteau’s reagent and gallic acid was used as standard. The methanolic extracts were also checked for primary antioxidant activities using an in vitro method, measured by free radical scavenging activity of 2, 2’-Diphenyl-1- Picrylhydrazyl (DPPH) radical. The absorbances were measured at 517 nm by UV/VIS spectrophotometer and compared with standards Trolox and Butylated Hydroxyanisole (BHA). The results from this study suggest that N. subdita heartwood extract may serve as a potential source of natural antioxidant for future development in food and nutraceutical applications.

Item Type:	Article (Journal)
Additional Information:	5678/26058
Uncontrolled Keywords:	2, 2' , antioxidant activity, Diphenyl-1-Picrylhydrazyl (DPPH) radical, metabolic extract, Nauclea subdita , TPC
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Jamaluddin Mohd Daud
Date Deposited:	30 Oct 2012 09:55
Last Modified:	20 Jun 2017 15:52
URI:	http://irep.iium.edu.my/id/eprint/26058

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