Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract

Bichi, Mustafa Hassan and Aguwamba, Jonah Chukwu and Muyibi, Suleyman Aremu and Abdulkarim, Mohd Ismail (2012) Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract. Journal of American Science, 8 (9). pp. 450-458. ISSN 1545-1003

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Abstract

Moringa Oleifera seeds extract has been found to posses antimicrobial properties. The mechanism of its action in this regard is still largely un-determined. In this work, six methods of extracting the active ingredients from the Moringa seed powder were considered. They were: Normal aqueous extraction (M1), Salt extraction (M2), Oil removal followed by aqueous extraction (M3), oil removal followed by salt extraction (M4), oil removal followed by aqueous extraction and microfiltration (M5), and oil removal followed by salt extraction and microfiltration. The antimicrobial action of each extract on Escherichia Coli was determined using Disc Diffusion and Agar Well methods. An average of 639.77±16.72gm of seed kernel was obtained per Kg of raw Moringa seed processed. The results also showed that 72.28±4.84% cake was produced for each 10gm of the <210 μm seed powder de-fatted and the seed oil produced was 28.75±1.68%. The zones of inhibition produced using disc diffusion method ranged 6.5mm – 9.0mm for the six extraction methods with the highest value of 9.0mm obtained with method M3. The Agar well method yielded 6.75mm – 12.38mm zone of inhibition diameter with the highest value of 12.38mm obtained with method M3 of seed extraction. The investigation thus concluded that the M3 was the best extraction method. The Minimum Inhibitory Concentration (MIC) for the Moringa disinfection Solution M3 was determined to be 200μL/mL while the Minimum Bacterial Concentration (MBC) was 210μL/mL. Optimization of the operating conditions was suggested for the subsequent application of this method of seed processing in water disinfection.

Item Type:	Article (Journal)
Additional Information:	4164/25909
Uncontrolled Keywords:	Moringa Oleifera, Seed Extract, Extraction Method, Disinfection, Antimicrobial Activity
Subjects:	T Technology > TD Environmental technology. Sanitary engineering T Technology > TD Environmental technology. Sanitary engineering > TD201 Water supply for domestic and industrial purposes
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof SULEYMAN AREMU MUYIBI
Date Deposited:	02 Oct 2012 08:24
Last Modified:	02 Oct 2012 08:24
URI:	http://irep.iium.edu.my/id/eprint/25909

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