Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka

Hashim, Ridzwan and Z., Farah Hanis and Mohd Daud, Jamaluddin and Althunibat, Osama Yousef (2012) Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka. European Journal of Scientific Research, 75 (2). pp. 255-261. ISSN 1450-216X, 1450-202X

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Abstract

Three local species of sea cucumbers; Holothuria edulis Lesson, H. scabra Jaeger and Stichopus horrens Selenka were studied for their body walls protein profiles using sodium dodecyl sulphate polyacylamide gel electrophoresis (SDS-PAGE). Their body walls were subjected to extraction process using lysis and resuspension buffers. The protein concentration was based on bovine serum albumin as standard. Analysis of the protein extract of the three species of sea cucumber revealed at least 11 type of proteins with molecular weight ranging from 20 kDa – 125 kDa. As for the protein profiles, significant difference in the protein binding patterns were noticed. The results showed the molecular weight of protein varied among species; 20 – 54 kDa for H. edulis and 54 – 125 kDa for H. scabra. However, protein fraction for S. horrens failed to be detected using this technique.

Item Type:	Article (Journal)
Additional Information:	5465/25253
Uncontrolled Keywords:	Sea cucumbers, Holothuria edulis, H. scabra, Stichopus Orrens Molecular Weight , Protein
Subjects:	Q Science > QK Botany
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Science
Depositing User:	prof Dr Ridzwan Hashim
Date Deposited:	09 Nov 2012 13:12
Last Modified:	09 Nov 2012 13:12
URI:	http://irep.iium.edu.my/id/eprint/25253

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