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Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare

Hashim, Yumi Zuhanis Has-Yun and Mel, Maizirwan and Mohd. Salleh, Hamzah and Ahmad Nor, Yusilawati and Othman, Siti Hajar and Wan Abdul Razak, Wan Yusra Hannanah (2012) Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare. Advances in Natural and Applied Sciences, 6 (5). pp. 596-600. ISSN 1995-0772

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Abstract

Serum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins, growth factors, hormones, vitamins, inorganic substances, nutrients and metabolites; which promote and sustain cell growth as well as provide buffering condition and protection to cells. Serum, from various animal sources such as bovine and porcine can be obtained commercially from manufacturers. Although serum has long been used successfully in mammalian cell culture system, recent trend is moving towards serum alternatives and serum-free media. This is due to many factors including the ethical and animal welfare issues in serum processing and production. Further, large scale production of bio-products using mammalian cell culture system prefers defined media which offers reproducibility and ease of downstream purification. Nevertheless, development of serum-free media is tedious and somewhat involve high cost with inherent problem of low growth rate as compared to serum supplemented media. This review is set to explore the many facets of serum and serum-free media in mammalian cell culture bioprocessing.

Item Type: Article (Journal)
Additional Information: 3774/24535
Uncontrolled Keywords: bioprocessing, ethical issues, mammalian cell culture, serum, serum-free media
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited: 18 Jul 2012 11:53
Last Modified: 14 Jan 2019 09:19
URI: http://irep.iium.edu.my/id/eprint/24535

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