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Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence

John, Akbar and Khan Chowdhury, Ahmed Jalal and Yunus, Kamaruzzaman and Kasim, Zaleha and Kumar, C. Prasanna (2010) Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence. In: 4th International Conference on Postgraduate Education (ICPE 4). , 26 - 28 November 2010, Kuala Lumpur, Malaysia.

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An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs, beetles, common crabs and scorpion sequences. Constructed phylogram showed beetles are closely related with horseshoe crabs than common crabs. Scorpion spp were distantly related to xiphosurans. Phylogram and observed genetic distance (GD) date were also revealed that Limulus polyphemus was closely related with Tachypleus tridentatus than with T.gigas. Carcinoscorpius rotundicauda was distantly related with L.polyphemus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L.polyphemus (38.32%) and lowest was observed in T.tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related species

Item Type: Conference or Workshop Item (UNSPECIFIED)
Additional Information: 3575/2404
Uncontrolled Keywords: Cytochrome C oxidase subunit I; DNA barcoding; Codon position; Horseshoe crabs.
Subjects: Q Science > QL Zoology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Kulliyyah of Science > Institute of Oceanography and Maritime Studies
Depositing User: Professor Ahmed Jalal Khan Chowdhury
Date Deposited: 25 Nov 2011 12:40
Last Modified: 06 Dec 2017 12:30
URI: http://irep.iium.edu.my/id/eprint/2404

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