Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement

Hussin, Akbar Sham and Sampson, Wayne John and Dreyer, Craig William and Pierce, Angela Mary and Ferguson, Ian Andrew (2009) Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement. Archives of Oral Biology, 54 (9). pp. 871-878. ISSN 0003-9969

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Abstract

Objectives Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of anti-NGF on these changes. Design Orthodontic separators were placed between the right maxillary first and second molars of Sprague–Dawley rats which were equally divided into two groups. Animals from the second group were injected with anti-NGF. The left sides served as controls, and animals were sacrificed at 0, 3, 7 and 14 days. Results Results of immunohistochemical localisation for p75, Trk A, calcitonin gene-related peptide (CGRP) and NGF showed staining intensity increased at day 3, with a peak at day 7 and decreasing intensity at day 14. Anti-NGF injected animals showed reduced staining at all observation periods. Conclusion Data suggest that orthodontic injury induces NGF production, leading to sprouting and invasion by CGRP-positive nerve fibers and that injection of anti-NGF reduces NGF tissue levels and prevents innervation by CGRP-positive fibers.

Item Type:	Article (Journal)
Additional Information:	5769/22589
Uncontrolled Keywords:	1.Nerve growth factor 2.Periodontium 3.Rat 4.Tooth movement
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry
Depositing User:	Mdm. Wahidah Mohd Zain
Date Deposited:	15 Mar 2012 16:55
Last Modified:	15 Mar 2012 16:55
URI:	http://irep.iium.edu.my/id/eprint/22589

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