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Effects of cell immobilization to the phytate-degrading enzyme activity

Mohamad Puad, Noor Illi and Farouk, Abd Alaziem and Salleh, Hamzah Mohd. (2011) Effects of cell immobilization to the phytate-degrading enzyme activity. In: Current Research and Development in Biotechnology Engineering at IIUM (volume II). IIUM Press, Kuala Lumpur, pp. 137-143. ISBN 978-967-418-151-2

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Abstract

The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroundings during immobilizalion and subsequent cominuous operalion. A baclerium isolated from Malaysian waslewatn pnxlucing a 1l0,-e1 phyrate-degrading enzyme namely PhyFAUIAI was immobilized in alginate gel beads. PhyFAUIA 1 is a periplasmatic enzyme and highly substralespecific 10 phytale. The enzyme aClivity wa~ invesligaled upon cell immobilization. No shift in pH oplima of phytase of immobilized cells was obsen·ed. 110wever. the optimum lcmperaturcbwas shifted 10 50°C, which was 15°C lower lhan free PhyFAUIAI enzyme. Immobilized cells were found 10 be more stable in wider range of pll and tcmpe-ramr.: compared to free cells. The results presenled in this work show lhe potential for using immobilized cells to produce ditrerent "~nJ-inosilOl phosphates intennediales

Item Type: Book Chapter
Additional Information: 1813/20776
Uncontrolled Keywords: Phytate degrading enzyme, phytase activity, cell immobilization, myo inositol, phosphates
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Noor Illi Mohamad Puad
Date Deposited: 27 Nov 2012 14:53
Last Modified: 27 Nov 2012 14:53
URI: http://irep.iium.edu.my/id/eprint/20776

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