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Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast

Che Seman, Che Nor Zarida and Othman, Fauziah and Moin, Saidi and Wong, C. C. and Besar, Idris and Mustaffa, Rusnah and Goriman Khan, Mohd Azam Khan and Ahmad, Azfar Rizal and Zulkifly, Ahmad Hafiz (2010) Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast. Malaysian Journal of Microscopy, 6 (1). pp. 1-8. ISSN 1823-7010

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Abstract

This study was carried out to observe the biocompatibility of gentamicin-coated hydroxyapatite (HA) on osteoblast using fluorescence microscopy. The specific objective was to observe the viability of the osteoblast on the gentamicin-coated hydroxyapatite (HA) and to determine the effect of the biomaterial coated with gentamicin on the osteoblast. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM's F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37°C in a 5% CO2. The cell lines were treated with different concentration of gentamicin-coated hydroxyapatite and the interactions of the antibiotic beads against osteoblast were tested using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The MTT assay results indicated that varying concentrations of gentamicin coated HA from 0.1 mg/ml to 10 mg/ml did not significantly affect viability of osteoblast. By employing fluorescence microscopy, the morphology of osteoblast observed appeared red in color which indicated that the osteoblast was viable on biomaterial. The pore size of hydroxyapatite is between 150 to 350 nm. This preliminary result suggested that the gentamicin-coated HA had a good biocompatibility towards osteoblast.

Item Type: Article (Journal)
Additional Information: 2397/19993
Uncontrolled Keywords: Biocompatibility; Fluorescence microscopy; Gentamicin-coated hydroxyapatite (HA); MTT assay; Osteoblast
Subjects: R Medicine > RD Surgery > RD701 Orthopedics
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User: Sr Che Nor Zarida Che Seman
Date Deposited: 15 Mar 2012 05:33
Last Modified: 17 Apr 2015 08:32
URI: http://irep.iium.edu.my/id/eprint/19993

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