Statistical modelling optimisation of cellulase enzyme immobilisation on functionalised multi-walled carbon nanotubes for empty fruit bunches degradation

Al-Khatib, Ma'an Fahmi Rashid and Alam, Md Zahangir and Mohammed, Rasha (2012) Statistical modelling optimisation of cellulase enzyme immobilisation on functionalised multi-walled carbon nanotubes for empty fruit bunches degradation. Australian Journal of Basic and Applied Sciences, 6 (1). pp. 30-38. ISSN 1991-8178

Preview

PDF Download (607kB) | Preview

Abstract

Cellulase obtained from the fermentation of sewage treatment plant sludge (STP) by Trichoderma-reesei RUT C-30 was covalently immobilised on functionalised multi-wall carbon nanotubes. Statistical optimisation using the Plackett–Burman design method was implemented to identify parameters with significant effects on the process of immobilisation. The results obtained from this Plackett–Burman design show that three parameters have a significant effect on immobilisation: pH, temperature, and N-ethyl-N-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) concentration. Based on our Plackett-Burman design results, these parameters were further optimised using a face-centred central composite design. The resulting optimum conditions for cellulase immobilisation, as determined by face-centred central composite design, were pH 4.5, 30°C, and 1 mL of 10mg/mL EDC. The amount of immobilised cellulase was approximately 98% using these optimum conditions. The resulting MWCNT-cellulase composite was further characterized by FTIR and SEM. The FTIR spectrum of MWCNT-cellulase composite showed an amide group peak (O = C-NH) corresponding to cellulase enzyme, which confirms that immobilisation took place.

Item Type:	Article (Journal)
Additional Information:	5595/17475
Subjects:	T Technology > TD Environmental technology. Sanitary engineering > TD172 Environmental pollution T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Ma'an Alkhatib
Date Deposited:	16 Feb 2012 14:17
Last Modified:	06 May 2024 14:34
URI:	http://irep.iium.edu.my/id/eprint/17475

Actions (login required)

View Item