Preliminary investigation of MYO-INOSITOL phosphates produced by ASUIA279PHYTASE on MCF-7 cancer cells

Yusoff, Narazah Mohd and Nuge, Tamrin and Zainan, Nur Hidayah and Hashim, Yumi Zuhanis Has-Yun and Jamal, Parveen and Meor Hussin, Anis Shobirin and Farouk, Abd Alaziem and Salleh, H.M. (2011) Preliminary investigation of MYO-INOSITOL phosphates produced by ASUIA279PHYTASE on MCF-7 cancer cells. IIUM Engineering Journal, 12 (4). pp. 1-11. ISSN 1511-788X

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Abstract

Phytate or myo-inositol hexakisphosphates (IP6) is widely distributed in plants like rice bran. The production of myo-inositol phosphate intermediates has received much attention due to the remarkable potential health benefits offered by the compounds. In this study, the cytotoxicity of the partially purified myo-inositol phosphate fractions and commercial IP1 and IP6 were investigated against MCF-7 breast cancer cell lines. The study showed that the commercial standard IP1 and IP6 showed good inhibition towards the MCF-7 cell line. The MCF-7 cells growth was inhibited in minimum concentration of myo-inositol phosphates (<1000 μg/ml). However, no inhibition observed on the MCF-7 cell line by the myo-inositol phosphates fractions partially purified from rice bran at concentration <1000 g/ml. The inhibition of MCF-7 was only observed at concentration more than 30 mg/ml with more than 40% cells were inhibited. This indicates that the partially purified rice bran myo-inositol phosphates degraded by ASUIA279 phytase on MCF-7 breast cancer cells exhibit positive results towards the inhibition of cancer cells growth at relatively high concentration.

Item Type:	Article (Journal)
Additional Information:	3774/17382
Uncontrolled Keywords:	myo-inositol phosphates; phytase; MCF-7cell line; cancer
Subjects:	Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Mrs Maznah Zakaria
Date Deposited:	30 Jan 2012 15:08
Last Modified:	30 Jan 2012 15:08
URI:	http://irep.iium.edu.my/id/eprint/17382

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