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Recovery of recombinant bromelain from Escherichia coli BL21-AI

Bala, M. and Salleh, Hamzah Mohd. and Amid, Azura and Mel, Maizirwan and Jami, Mohammed Saedi (2011) Recovery of recombinant bromelain from Escherichia coli BL21-AI. African Journal of Biotechnology, 10 (81). 18829-18832. ISSN 1684–5315

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Abstract

In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombinant bromelain was expressed as soluble (active) and insoluble (inactive) enzyme in Escherichia coli BL21-A1. The enzyme fractions were purified using Ni-NTA His•Bind resin with the aid of an automated fast protein liquid chromatography (FPLC) system. Purification fold and percentage yield of the purified active bromelain were found to be 3.7 and 64%, respectively. SDS-PAGE results showed that the enzyme was purified to near homogeneity and the soluble form exhibited a single band with molecular weight of about 45 kDa.

Item Type: Article (Journal)
Additional Information: 1813/14293
Uncontrolled Keywords: bromelain, Ni-NTA His•Bind resin, fast protein liquid chromatography (FPLC), ananas comosus
Subjects: T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Nur' Aini Abu Bakar
Date Deposited: 05 Mar 2012 14:28
Last Modified: 05 Mar 2012 14:28
URI: http://irep.iium.edu.my/id/eprint/14293

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