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In silico approach in designing Xylanase for biobleaching industry

Noorbatcha, Ibrahim Ali and Abdul Hadi, Muaz and Ismail, Ahmad Faris and Hamzah, Mohd. Salleh (2009) In silico approach in designing Xylanase for biobleaching industry. In: 3rd International Conference on Chemical & Bioprocess Engineering , 12–14 August 2009, Kota Kinabalu, Sabah.

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Abstract

The use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatures and higher alkaline pH conditions. In this work we have used computational methods to analyze the structural factors responsible for the activity and themostability of Bacillus circulans xylanase which have been identified as one of the promising xylanase source to treat the pulp before bleaching it through the conventional bleaching sequences. Simulated point mutation shows that arginine substitution potentially increased the number of hydrogen bond; correlate with the xylanase activity and some changes in amino acid sequence specifically to the α-helix and β-sheet appeared to be promising in improving thermostability of xylanase. This in silico method results can be used to develop new efficient xylanase for pulp bleaching industry.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 3704/13910
Uncontrolled Keywords: Bacillus circulans xylanase, in silico mutagenesis, enzyme mutation, point mutation, thermostable xylanase
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Depositing User: Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited: 15 Jan 2014 11:30
Last Modified: 15 Jan 2014 11:30
URI: http://irep.iium.edu.my/id/eprint/13910

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