Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases

W. S. , Wan Muhammad Salahudin and Tun, Ye and Zulkifly, Ahmad Hafiz (2011) Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases. In: 20th Scientific Conference of the Microscopy Society of Malaysia & 21st Annual General Meeting, 20-22 December 2011, Port Disckson, Negeri Sembilan. (Unpublished)

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Abstract

Diabetes mellitus causes several changes in fiber size and oxidative capacity in skeletal muscle. Gastrocnemius, Soleus and Anterior Tibialis muscle are important leg muscles involve in reconstruction of amputation stump. The fiber size and oxidative enzyme are important factors related to the viability of muscle and subsequently stump healing. Aim of the study: To describe the fiber size and oxidative capacity quantitatively in 3 muscles of the leg Materials and methods: Specimen obtained from the 10 diabetes mellitus subjects undergone below knee amputation (BKA). Specimen was stained for enzyme histochemistry NADH-TR staining. The slide was analyzed with ImageJ software for fiber type distribution and oxidative capacity based on staining density. Result and discussion: Gastrocnemius muscle has predominantly Type 1 muscle fibers and highest oxidative capacity, while the anterior tibialis muscle has predominantly Type 2 muscle fibers and lowest oxidative capacity. Soleus muscle has almost equal Type 1 and 2 muscle fibers and has intermediate oxidative capacity. Conclusion: Muscle with predominant type 1 fibers has highest oxidative capacity.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	3693/13782
Uncontrolled Keywords:	oxidative capacity, diabetes mellitus, skeletal muscle
Subjects:	Q Science > QM Human anatomy
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User:	Dr Ye Tun
Date Deposited:	16 Feb 2012 12:37
Last Modified:	21 Jun 2017 14:55
URI:	http://irep.iium.edu.my/id/eprint/13782

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