Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology

Azmi, Azlin Suhaida and Ngoh, Cheng G. and Mel, Maizirwan (2011) Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology. African Journal of Biotechnology, 10 (81). pp. 18833-18841. ISSN 1684–5315

Preview

PDF (Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology) - Published Version Download (412kB) | Preview

Abstract

Ethanol production by co-culture of ragi tapai and Saccharomyces cerevisiae from unhydrolyzed cassava starch without addition of enzymes was conducted in a 2 L batch fermentor. Taguchi’s method with orthogonal array of L8 was applied in design of experiment (DOE) and the results were analyzed using MINITAB v14 software. Seven factors: Nitrogen-phosphorus-potassium (NPK), urea, fermentation temperature, ragi tapai concentration, S. cerevisiae concentration, agitation and co-culturing time were varied at two levels for each factor. The significant factors for the production of ethanol were determined by setting S/N ratio to “larger-is-better” for high yield ethanol and “smaller-is-better” for low yield of ethanol byproducts. The optimum values obtained for each factor were similar to each other. Both have optimum factors of: Urea at 0.8 w/w%, dry ragi tapai and S. cerevisiae concentrations each at 10 w/w%, co-culturing time at 3 h gap, NPK at 0.09 w/w% and agitation speed at 100 rpm. The fermentation temperature for high ethanol yield was 35°C, whereas for the byproducts was 30°C. From the validation experiment conducted at 30°C in 10 L fermentor, the ethanol concentration obtained was 68.00 g/L, while all byproducts concentrations were below 9.00 g/L at the end of the fermentation.

Item Type:	Article (Journal)
Additional Information:	5339/13696
Uncontrolled Keywords:	co-culture fermentation, ethanol, Taguchi’s experimental design, ragi tapai, Saccharomyces cerevisiae.
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	04 Jan 2012 15:06
Last Modified:	04 Nov 2012 16:40
URI:	http://irep.iium.edu.my/id/eprint/13696

Actions (login required)

View Item