Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation

Azzubaidi, Marwan Saad and Saxena, Anil Kumar and Talib, Norlelawati A. (2011) Quantifying dorsal hippocampal CA1 pyramidal cells in rats: Rules to Light Microscope based estimation. In: Harnessing Microscopy and Nuclear Science for Green Technology, 20-22 December 2011, Port Dickson, Malaysia.

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Abstract

The brain’s hippocampus which controls memory and learning is the first structure affected by neurodegeneration in Alzheimer’s disease (AD). The number of viable hippocampal CA1 pyramidal cells (HCA1PC) is significantly decreased in AD and in rats with 2 vessel occlusion (2VO), an established animal model for AD. The variability in three dimensional arrangements of HCA1PC between dorsal and ventral portions of the hippocampus made the calculation of HCA1PC subject to debate. As errors and biases in the counting technique can be overcome by implementing a standardized procedure, the current study proposed a procedure for quantification of the viable HCA1PC by standardizing the method of tissue sectioning. Eight rats were divided into 2VO and sham control with four animals per group. Coronal sections were made based on the anatomical landmarks of medial section of rat’s cerebral hemispheres. Under light microscope with cresyl violet staining, the number of viable HCA1PC cells in 1 mm horizontal distance of dorsal hippocampus, 3 mm caudal to the fornix, was calculated twice and found to be significantly higher in sham control than 2VO group (p<0.01) with standard error of mean of 4.98 and 17.26 respectively. This indicates successful establishment of the standardized technique with feasible reproducibility. 1 International Islamic University Malaysia, Faculty of Medicine Department of Basic Medical Sciences

Item Type:	Conference or Workshop Item (Full Paper)
Additional Information:	5517/13515
Uncontrolled Keywords:	Hippocampus, pyramidal cells, Alzheimer, 2VO, neurodegeneration,rats
Subjects:	R Medicine > RC Internal medicine > RC321 Neuroscience. Biological psychiatry. Neuropsychiatry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Dr. Anil Kumar Saxena
Date Deposited:	14 Mar 2012 15:39
Last Modified:	17 Dec 2012 14:23
URI:	http://irep.iium.edu.my/id/eprint/13515

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