Radiosensitivity test on two varieties of Terengganu and Arab used in mutation breeding of roselle (Hibiscus sabdariffa L.)

S.S, Harding and Osman, Mohamad (2009) Radiosensitivity test on two varieties of Terengganu and Arab used in mutation breeding of roselle (Hibiscus sabdariffa L.). African Journal of Plant Science, 3 (8). pp. 181-183. ISSN 1996-0824

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Abstract

A study was carried out in a greenhouse to determine the effective doses for mutation breeding of roselle during the year 2006/2007. Seedling height was the parameter recorded at 2, 3, 4 and 5 weeks after sowing of M1 seeds of 2 varieties Terengganu and Arab. The results indicated that increasing doses of gamma irradiation from 0 to 1200 Gy in steps of 100 Grey (Gy) caused increasing physiological effect on seedling height. There was a progressive increase in seedling height from 2 until 5 weeks after sowing. However, at week 2 and 3 after sowing there was no significant difference between seedling heights. The LD50 values determined from regression analyses for Terengganu based on seedling height were 754, 821.4, 761.7 and 766.7% at 2, 3, 4 and 5 weeks after sowing, respectively and the LD50 values for Arab were 773.8.%, 804.1.%, 704.2 and 708.3% at 2, 3, 4 and 5 weeks, respectively. 2 weeks after sowing appears to be the most appropriate time for data collection. The LD50 values at 2 weeks were not significantly different from 3, 4 and 5 weeks after sowing. The LD50 values for Terengganu and Arab determined at 2 weeks were 754 and 773.8%, respectively.

Item Type:	Article (Journal)
Additional Information:	6408/12689
Uncontrolled Keywords:	Roselle, mutation breeding, seedling height, LD50, radiosensitivity, dosimetry, Hibiscus sabdariffa, gamma irradiation
Subjects:	S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Prof. Dr. Mohamad Osman
Date Deposited:	05 Jan 2012 13:20
Last Modified:	26 Jan 2012 10:39
URI:	http://irep.iium.edu.my/id/eprint/12689

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