The impact of supplementing Lactococcus lactis as probiotics on zebrafish (Danio rario) upon beıng infected Vibrio parahaemolyticus

Khairul Anuar, Khairul Danish Hafizi and Mohd Nasir, Mohd Hamzah and Tengku Abdul Hamid, Tengku Haziyamin (2025) The impact of supplementing Lactococcus lactis as probiotics on zebrafish (Danio rario) upon beıng infected Vibrio parahaemolyticus. In: 14th International Molecular Biology and Biotechnology Congress, 29th December 2025, Online Conference.

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Abstract

A recently isolated Lactic acid bacteria (LAB) strain Lactococccus lactis was found to antagonize a fish and shrimp pathogen Vibrio parahaemolyticus (VP). This probiotic strain was applied on juvenile zebrafish (Danio rerio) feeding, in order to understand the effect of probiotic strain on young and growing fish. Zebrafish larva at 10dpf were fed with probiotics until 40dpf, and subjected to infection challenge with VP. Four zebrafish’s group were subjected to 4 different treatments: Control, A, B and C. The Control group was given neither probiotic treatment, nor being subjected to VP infection; Group A had probiotic treated without infection; Group B had no probiotic treatment but with infection; and Group C was infected without probiotic treatment. For the infected group (B and C), infection challenges were carried out at 40dpf, and fish survivability, growth performance in term of feed conversion ratio (FCR), the body weight and body length gains were measured at 43 dpf. Results have shown that the survivability rate was 80±7% for Control group; 75±5% for Group A (probiotic treated but no VP infection), and 55±6% for Group B (infected group treated with probiotics). Infected group without probiotics treatment (C) showed no survival. At p< 0.05, treatment A, B and C recorded the higher body weight gain than A, and Control group gave the lowest. In terms of body length gain, treatment B was the highest (~0.88%), while Control group was the lowest (0.52%). This study demonstrated that L. lactis treated as probiotics on zebrafish was able to enhance its growth and survivability during VP infection.

Item Type:	Proceeding Paper (Other)
Additional Information:	5522/126759
Uncontrolled Keywords:	Zebrafish, Growth performance, Probiotic, Vibrio parahaemolyticus, Lactococcus lactis
Subjects:	Q Science > Q Science (General) S Agriculture > SH Aquaculture. Fisheries. Angling
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited:	09 Jan 2026 11:46
Last Modified:	09 Jan 2026 11:47
Queue Number:	2026-01-Q1572
URI:	http://irep.iium.edu.my/id/eprint/126759

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