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Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment

Alfaqeh, Hamoud Hussein Mohammed and Kienhui, Chua and Bin Saim, Aminuddin and Bint Haji Idus, Ruszymah (2008) Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment. In: 2nd Malaysia Tissue Engineering and Regenerative Medicine 22-23 July 2008 , 22-23 July 2008, HUKM .

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Abstract

Abstract: Objectives: This study was aimed to examine the effect of two different media on bone marrow stem cells (BMSCs) chondrogenesis in vitro and in vivo. Methods: BMSCs were isolated from sheep and divided into two test groups and control group. Control group was cultured in medium supplemented with 10 % fetal bovine serum (FBS). Test group A was cultured in medium supplemented with 1% FBS, 1% ITS, 5ng/mL IGF-1, 3ng/mL bFGF and 1ng/mL TGF-β2. Test group B was cultured in medium supplemented with 1%FBS, 1% ITS, 5ng/mL TGF-β3, 50ng/mL IGF-1, 40 ng/mL L-proline, 100 nM dexamethasone and 50 μg/mL ascorbic acid-2 phosphate. The experiment was carried out in Institute of Medical Research (IMR) and Universiti Kebangsaan Medical Centre Malaysia, Kuala Lumpur city, from 19 April 2007 to 25 December 2008. Results: Cell aggregates were formed in test group A and more in test group B denoted the early chondrogenesis in culture. Later, cells-fibrin constructs were made and implanted subcutaneously into nude mice for 5 weeks, then explanted for histological examination and glycosaminoglycans (GAG) quantification. Test group B constructs showed highest cartilage matrix synthesis confirmed by Safranin O staining and GAG production. Conclusion: These results demonstrated the effectiveness of medium supplemented with TGF-β3 in the combination to promote chondrogenesis in BMSCs.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 6697/12551
Uncontrolled Keywords: Stem cells, Chondrogenesis, Growth factors, Cartilage, Bone marrow, differentiation, glycosaminoglycans
Subjects: Q Science > QP Physiology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Dentistry
Depositing User: Dr Hamoud Alfaqeh
Date Deposited: 27 Dec 2012 14:11
Last Modified: 27 Dec 2012 14:11
URI: http://irep.iium.edu.my/id/eprint/12551

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