Pro-apoptotic effects of dithiocarbamate on human oral cancer cell lines

Suriyah, Wastuti Hidayati and How, Fiona Ni Foong and Kasmuri, Abdul Razak and Md Isa, Muhammad Lokman and Ichwan, Solachuddin Jauhari Arief (2025) Pro-apoptotic effects of dithiocarbamate on human oral cancer cell lines. In: Current Research and Trends in Dental and Medical Technology. CRC Press, London, pp. 449-456. ISBN 9781041088738

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Abstract

Objectives: To investigate the anticancer potential of dithiocarbamate (DTC) on oral cancer cells. Methods: Human oral squamous carcinoma cell lines, HSC-3 and HSC-4 were used to assess cytotoxicity by MTT assay. Apoptosis was evaluated by flow cytometry analysis, while the expression of pro-apoptotic genes PUMA and Caspase-9 was measured using quantitative RT-PCR. Data were analyzed using one-way ANOVA and Dunnett’s test to compare treatment and control groups. Results: DTC reduced the viability of oral cancer cells in a dose-and time-dependent manner. Flow cytometry revealed an increase in apoptotic cells, which was supported by a significant upregulation of PUMA and Caspase-9 (p<0.05). Conclusion: DTC demonstrates significant pro-apoptotic activity against HSC-3 and HSC-4 cell lines, highlighting its potential as a novel chemotherapeutic agent.

Item Type:	Book Chapter
Uncontrolled Keywords:	Apoptosis, Dithiocarbamate, HSC-3, HSC-4, OSCC
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry Kulliyyah of Science Institute of Planetary Survival for Sustainable Well-Being (PLANETIIUM)
Depositing User:	Dr Fiona How
Date Deposited:	08 Dec 2025 16:35
Last Modified:	08 Dec 2025 16:35
Queue Number:	2025-12-Q642
URI:	http://irep.iium.edu.my/id/eprint/125055

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