Oral bacteria composition between healthy and mild cognitive impairment geriatric patients using next-generation sequencing

Mohd Nazri, Nur Sabrina Ameerah and Shalihin, Mohd Shaiful Ehsan and Hamzah, Hairul Aini and Abidin, Sulhi and Mohammad Aidid, Edre and Musa, Ramli (2025) Oral bacteria composition between healthy and mild cognitive impairment geriatric patients using next-generation sequencing. In: International Biomedical Conference 2025, 2025, Kuantan, Pahang, Malaysia.

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Abstract

INTRODUCTION: Disruptions in the oral microbiome, particularly bacterial communities, have been linked to systemic inflammation and neurodegenerative diseases, including mild cognitive impairment (MCI). However, the oral bacterial profile of elderly individuals with MCI remains underexplored. OBJECTIVES: This study aimed to compare the oral bacterial composition of healthy and MCI elderly individuals using next-generation sequencing. MATERIALS & METHODS: A total of 17 geriatric participants were recruited, including healthy (n = 8), MCI (n = 8), and one with overt dementia. Dental plaque (n = 11) and saliva (n = 6) were collected. DNA extraction was followed by 16S rRNA sequencing targeting the V3–V4 region using the Illumina platform. Bioinformatic analysis assessed alpha and beta diversity and taxonomic composition of oral bacteria. RESULTS: Alpha diversity (Shannon, Chao1, and ACE indices) was slightly higher in the MCI group, though not statistically significant. Beta diversity analysis showed overlapping bacterial communities, with no significant separation between groups based on PERMANOVA (R² = 0.124, p = 0.515). Most bacterial genera did not show large or consistent differences between groups. However, Streptococcus, Treponema, Prevotella_7, and Neisseria appeared more abundant in the healthy group, while Veillonella, Fusobacterium, Leptotrichia, and Porphyromonas were more abundant in the MCI group, although inter-individual variability was observed. CONCLUSION: Preliminary findings suggest subtle differences in oral bacterial diversity and composition between MCI and healthy elderly individuals. Further analysis with a larger sample is underway to validate these trends.

Item Type:	Proceeding Paper (Poster)
Additional Information:	5342/124551
Uncontrolled Keywords:	Oral Bacteria, Mild Cognitive Impairment, Geriatric, 16S rRNA Sequencing, Microbial Diversity
Subjects:	Q Science > QR Microbiology R Medicine > RC Internal medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Prosthodontics Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine > Department of Community Medicine (Effective: 1st January 2011) Kulliyyah of Medicine > Department of Family Medicine (Effective: 1st January 2011) Kulliyyah of Medicine > Department of Psychiatry
Depositing User:	Dr Hairul Aini Hamzah
Date Deposited:	25 Nov 2025 14:54
Last Modified:	25 Nov 2025 15:02
Queue Number:	2025-11-Q111
URI:	http://irep.iium.edu.my/id/eprint/124551

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