Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines

Maghdooni Bagheri, Pegah and Rahman, Mohammad Tariqur and Van Soest, Sofie and De Ley, Marc (2009) Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines. Journal of Trace Elements in Medicine and Biology, 23 (2). pp. 124-31. ISSN 0946-672X (P), 1878-3252 (O)

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Abstract

The expression pattern of functional members of the metallothionein (MT) gene family was studied in the haematopoietic precursor cell lines, K562, DAMI, MEG-01, and ELF-153 in order to strengthen the proposed function of MT in differentiation. Cells were cultured in RPMI 1640 with 10% (v/v) foetal calf serum, with or without different zinc supplements. Expression of MT isogenes was analysed by quantitative real-time PCR (RT-PCR) using mRNA extracted from cultured cells. The more mature K562, DAMI, and MEG-01 cell lines exhibited transcription of all MT isogenes, except MT-3 and MT-4. Relative quantitative expression of MT isogenes in the mature cell lines such as K562, DAMI, and MEG-01 was higher than in the immature ELF-153 cell line. Immunohistochemical staining (IHC) reveals an increased MT protein biosynthesis in more mature cell lines such as K562, DAMI and MEG-01 greater than in the immature ELF-153 cell line. Real-time PCR and immunohistochemical staining for investigating the effect of phorbol ester and hemin (haematopoietic differentiation stimuli) on expression of MT isogenes in K562 cells reveals that phorbol ester induces increased MT transcription and biosynthesis. Therefore, to our knowledge, the role of MT in differentiation in human haematopoietic precursor cell lines is here reported for the first time.

Item Type:	Article (Journal)
Additional Information:	5128/12434
Uncontrolled Keywords:	Haematopoietic differentiation; Megakaryocytic cell lines; Metallothionein; Phorbol ester; Zinc
Subjects:	Q Science > QP Physiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Mohammad Tariqur Rahman
Date Deposited:	03 Jan 2012 10:09
Last Modified:	01 Feb 2012 11:29
URI:	http://irep.iium.edu.my/id/eprint/12434

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