Aggregation and biofilm formation of mono- and co-culture Candida species and Staphylococcus aureus are affected by nutrients in growth media

Wan Ahmad Kamil, Wan Nurhazirah and Zainal, Mukarramah and Mokhtar, Munirah and Bandara, H.M.H.N and Dashper, Stuart G. and Arzmi, Mohd Hafiz (2025) Aggregation and biofilm formation of mono- and co-culture Candida species and Staphylococcus aureus are affected by nutrients in growth media. Biofouling, 41 (6). pp. 1-15. ISSN 0892-7014

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Abstract

Candida species and Staphylococcus aureus coexist in nosocomial infections. These interkingdom interactions are associated with oral biofilm formation, leading to various oral diseases. This study elucidated the interkingdom interactions of these microorganisms, particularly their aggregation and biofilm formation, in three different media. Candida auris, Candida albicans, Candida lusitaniae, Candida dubliniensis, Candida parapsilosis, Candida glabrata and S. aureus were used in this study. Aggregation assays were conducted to determine planktonic interaction, and biofilm assays were performed to investigate intra- and interkingdom interactions in a static biofilm environment. Most Candida spp. exhibited a high auto aggregation percentage in brain heart infusion broth supplemented with yeast extract (BHIYE). In addition, co-culture biofilm with S. aureus significantly reduced the total cell counts of Candida spp. compared to mono-culture (p<0.05). In conclusion, co-aggregation, biofilm biomass and total cell count were species- and growth medium-dependent, and S. aureus interacted antagonistically with Candida spp.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Interkingdom interactions; co-infections; growth media; aggregation; biofilm; Candida
Subjects:	Q Science > QR Microbiology R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry
Depositing User:	AP Ts Dr Mohd Hafiz Arzmi
Date Deposited:	15 Jul 2025 12:47
Last Modified:	15 Jul 2025 12:47
URI:	http://irep.iium.edu.my/id/eprint/122048

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