Box-behnken design and molecular docking assisted quenching spectrofluorimetric method for the quantitation of citalopram HBr in commercial dosage forms

Azmi, Syed Najmul Hejaz and Nasir Al Rawahi, Wafa Aqib and Zahran Al Nabhani, Zamzam Sulaiman and Ahmed Abdullah Al Farsi, Al Anood and Ahmed, Qamar Uddin and Alam, Mahboob and Mia, Abdur Rashid and Rahman, Nafisur (2025) Box-behnken design and molecular docking assisted quenching spectrofluorimetric method for the quantitation of citalopram HBr in commercial dosage forms. Journal of Fluorescence. pp. 1-13. ISSN 1053-0509 E-ISSN 1573-4994

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Abstract

A simple, sensitive and accurate spectrofluorimetric method was presented for the determination of citalopram hydrobromide (CTM) in tablets. The method was based on the quenching of bovine serum albumin (BSA) fluorescence with CTM at pH 7.4. The fluorescence intensities were measured at 342 nm after excitation at 279 nm. Various factors affecting the quenching of BSA fluorescence were optimized by response surface methodology (RSM) through Box-Behnken design (BBD. The plot of F0/F versus CTM concentration was linear in the concentration range of 10–100 µg mL-1. The limit of detection (LOD) and limit of quantitation (LOQ) were 2.08 and 6.30 µg mL-1, respectively The molar combining ratio between CTM and BSA was 1:1. The complementary modified green analytical procedure index (ComplexMoGAPI) was 90%. The effect interference of common excipients found in tablets was investigated. Percent recoveries of CTM was ranged from 99.92 to 100.27% in tablets.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Citalopram HBr · Quenching · Box-Behnken design · Molecular docking · ComplexMoGAPI
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Qamar Uddin Ahmed
Date Deposited:	29 May 2025 08:24
Last Modified:	29 May 2025 08:27
URI:	http://irep.iium.edu.my/id/eprint/120485

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