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A Cryopreservatlon method of Sclzaphyllurn commune local strains

Mohd Kamal, Irnada Syuhaidah and Mohd Nor Rashid, Noraswati and Mel, Maizirwan (2011) A Cryopreservatlon method of Sclzaphyllurn commune local strains. In: International Congress of the Malaysian Society for Microbiology 2011, 8-11 December 2011, Penang.

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Schizophyllan commune fsolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature. After the grains were fully covered with myceHa, they were place into a cryo-vial with three different. treatments, 10% glycerol, sterile distilled water and no cryoprotectants. AU of the treatments were frozen in liquid nitrogen for 7 days followed by thawing (cooling) at 30°C for 30 min. The grains were inoculated onto PDA agar to observe the viability (rate of survival) of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100% viability aft.er 48 hours and wheat wit.h no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study, a cryopreservation process was simplified as opposed to the conventional method in which 10% glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservalion which is more economical as no cryoprotectants were required.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4809/11991
Uncontrolled Keywords: Cryopreservation, Sclzaphyllurn,
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Depositing User: Dr Maizirwan Mel
Date Deposited: 31 Mar 2012 07:35
Last Modified: 09 Sep 2015 11:05
URI: http://irep.iium.edu.my/id/eprint/11991

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