Tualang Honey formulation as potential osteoarthritis treatment: in-vitro genotoxicity outcome

Abd Rahim, Nour El Huda and Zulkifly, Ahmad Hafiz and Mohd Jan, Nurul Hafiza and Ibrahim, Mohd Zulfadzli and Mohamad Amri, Nur Fathonah and Hassan, Habibah and Rajab, Nor Fadilah (2024) Tualang Honey formulation as potential osteoarthritis treatment: in-vitro genotoxicity outcome. Malaysian Orthopaedic Journal, 17 (Suppl. A). p. 1. ISSN 1985-2533 E-ISSN 2232-111X

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Abstract

INTRODUCTION: Osteoarthritis (OA) is a degenerative joint disease affecting millions worldwide. In the orthopaedic field, viscosupplementation is used as an option treatment for pain relief among osteoarthritis patients. Tualang Honey Formulation (THF) (Patent no. MY179303-A) is currently being studied as a viscosupplement option for OA treatment. This study aimed to investigate the cell mutagenic effect after exposure to THF towards biocompatibility profile. MATERIALS & METHODS: The bacterial tester strains (TA98, TA100, TA1535, TA1537 and WP2) were used to evaluate the mutagenicity of different concentrations of THF in the presence and absence of metabolic enzymes. The negative control was 0.9% NaCl. The positive controls were 4-nitro-o-phenylenediamine and 2-aminoanthracene. A total of 0.1ml of each concentration of THF was mixed with 0.5ml of buffer/S9 mix, followed by the addition of 0.1ml of each tester strain suspension. The mixture was then shaken in a water bath for 20-30 minutes at 37°C. The samples then were incubated at 37°C for 48 hours. The revertant colonies then were counted. RESULTS AND DISCUSSIONS: Figure 1 shows the number of colonies grown on the plates (revertant bacterial colonies counts) in all strains. The number of revertant colonies treated with the THF did not exceed twice the number of the negative control either in the presence or absence of metabolic activation. The negative and positive controls used in this study responded as expected. The outcome of this study showed that THF did not demonstrate a mutagenic effect on all bacterial strains, which indicated that THF could be used safely as a viscosuppplement in OA treatment. CONCLUSION: Based on the findings of this study, THF is a non-mutagenic and biocompatible. This outcome shows that THF is a potential viscosupplement for OA treatment in future.

Item Type:	Article (Abstract)
Additional Information:	6300/116543
Uncontrolled Keywords:	Tualang Honey, Osteoarthritis, Genotoxicity
Subjects:	R Medicine > R Medicine (General) R Medicine > RD Surgery R Medicine > RD Surgery > RD701 Orthopedics
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User:	Dr Nour El Huda Abd Rahim
Date Deposited:	10 Dec 2024 15:09
Last Modified:	10 Dec 2024 15:20
URI:	http://irep.iium.edu.my/id/eprint/116543

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