In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy)

Anisya, Shifa Nur and Nisa, Fithri Choirun and Liew, Kai Bin and Helal Uddin, A.B.M. (2024) In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy). Malaysian Journal of Chemistry, 26 (5). pp. 424-430. ISSN 1511-2292 E-ISSN 2550-1658

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Abstract

P. macrocarpa is often known as “God’s crown” due to its great nutritional importance for human health. P. macrocarpa fruit can delay and avoid oxidative cell damage in the body by its antioxidant properties. In the meantime, there is still lacking yield, antioxidant activity, and functional properties of P. macrocarpa fruit in the sonication method using water and ethanol. So, this study aims to compare P. macrocarpa fruit yield, and antioxidant properties and to identify its functional group. Crude ethanol extract and water extract of P. macrocarpa fruit have been studied for Ferric Reducing Antioxidant Power (FRAP) and 2,2- Diphenyl-1- Picrylhydrazyl (DPPH) antioxidant activities against Ascorbic acid as a standard. The study also determined the functional group using FTIR (Fourier Transform Infrared Spectroscopy) assay. The most effective P. macrocarpa fruit yield was obtained in the water extract compared to the ethanol extract. In addition, the ethanol extract showed a higher FRAP (54.285±1.139 mgAAE/g) effect than the water extract. Besides, the DPPH activity of ethanol extract (46.113±1.535 µg/mL) was higher than water extract (59.406±2.089 µg/mL) and lower than standard Ascorbic acid (8.287±0.125 µg/mL). Moreover, some functional groups such as -OH, aliphatic CH, aromatic CH, aromatic C=C and C=N were also identified in both P. macrocarpa fruit extracts. The results confirmed that the sonication method using ethanol and water could influence the yield of P. macrocarpa fruit extracts as well as antioxidant activities with different functional groups.

Item Type:	Article (Journal)
Uncontrolled Keywords:	P. macrocarpa fruit, extraction process, antioxidant activity, functional groups, FTIR
Subjects:	Q Science > QD Chemistry R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. A. B. M. Helal Uddin
Date Deposited:	05 Dec 2024 12:17
Last Modified:	05 Dec 2024 12:20
URI:	http://irep.iium.edu.my/id/eprint/116370

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