Repeated blood sampling from an adult Danio Rerio

Eissa, Manar and Abdullah, Raihan and Sharif, Faez and Mohd Nasir, Mohd Hamzah (2024) Repeated blood sampling from an adult Danio Rerio. In: Zebrafish as a Model for Parkinson’s Disease. CRC Press, Boca Raton, pp. 75-87.

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Abstract

Zebrafish (Danio rerio) has emerged as an enormously powerful and popular model for biomedical and preclinical research over the past few years. This is largely attributed to the significant genetics and physiological homology with higher vertebrates, especially humans, and the emerging opportunities for genetic manipulation and live in vivo imaging. Furthermore, the body fluids of zebrafish, including blood, plasma, and interstitial and cerebrospinal fluids, can also provide an insightful data on health and disease status. The high similarity between zebrafish and human plasma proteins makes zebrafish an excellent alternative model organism for studying hematologic and metabolic diseases in the quest for a new therapy. The analysis of the metabolites and the blood parameters of zebrafish is essential, as it might provide important details about the physiological condition of the fish. Besides, the longitudinal analysis of individual zebrafish provides a better insight of metabolic dynamics than the single point blood analysis. Despite the advantages of employing zebrafish for metabolic and blood research studies, it can be difficult to collect blood samples from these tiny aquatic creatures, owing to the fact that a 4-cm-long adult zebrafish only has about 0.05–0.07 ml of blood. As a result, several techniques for routine blood collecting from adult zebrafish have been developed. These techniques include decapitation, lateral incision in the dorsal aorta, tail-clipping, and heart puncture. Some of these techniques have been deemed as invasive or even harmful, whereas others are non-invasive and safe to employ for repeated blood samples from the same fish. The various blood collection techniques are briefly compared in this chapter, along with their benefits and drawbacks. It is important to keep in mind that some blood sampling techniques are not better than others; instead, the best techniques should be chosen based on the objectives of the study and the bare minimum quantity of blood needed for analysis. This chapter will also introduce a new and low-cost instrument that can be used for repeated blood sampling from adult zebrafish for blood glucose monitoring, with a survival rate of 93%. The fabricated instrument comprised a pulled microcapillary glass needle with an outer tip diameter of about 20 µm. The needle is inserted at the caudal vein, where 5–7 µl of blood were drawn at 30 minutes to 1-hour time intervals. This proposed device can be simply fabricated in new labs for drawing blood from zebrafish with high survival rate and at minimal cost.

Item Type:	Book Chapter
Uncontrolled Keywords:	Danio rerio, caudal vein, blood
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Mohd Hamzah Mohd Nasir
Date Deposited:	04 Jan 2025 21:09
Last Modified:	04 Jan 2025 21:38
URI:	http://irep.iium.edu.my/id/eprint/116115

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