Abd Rahman, Izzat Zulhilmi and Wan Omar, Wan Fatein Nabeila and Abdullah, Aszrin (2024) Urine metabolomics in dengue infection: elucidating hexadecane as a potential biomarker. Medicine & Health, 19 (5 (Suppl.)). p. 52. ISSN 1823-2140 E-ISSN 2289-5728
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Abstract
Introduction: Dengue infection remains a major global health concern, necessitating rapid, non-invasive, and universally accessible diagnostic methods. Existing diagnostic modalities fail to simultaneously address the critical aspects of sensitivity, specificity, efficiency, and cost-effectiveness. The NS1 antigen detection test exhibits low sensitivity, while serology tests carry a high risk of false negativity due to low IgM titres in the early phase of infection. Urine-based metabolic profiling has emerged as a promising approach for the identification of specific disease biomarkers. This study aims to identify urinary metabolites associated with dengue infection, leveraging these biomarkers to develop a urine-based dengue test kit. Alkane group metabolites may rise as they act as energy substrate to assist viral replication. Materials and method: We analysed urine samples from 30 dengue-infected patients and 30 control subjects using Gas Chromatography-Mass Spectrometry (GC-MS). The analysis focused on identifying distinct metabolites based on retention time (RT) and similarity index (SI), with the quantification of their concentrations using peak area. Results: Our findings revealed a significant presence of hexadecane in the urine samples of dengue-infected subjects (p<0.01) compared to control subjects. This metabolite was characterized by a RT of 20.95 + 2.23 minutes, a SI of 85.50 + 5.00%, and a peak area of 1360566.25 + 1066618.37 a.u. Conclusion: The marked detection of hexadecane specifically in dengue patients, highlights its potential as a key biomarker for dengue detection. This discovery establishes the foundation for the development of rapid, non-invasive urine-based dengue diagnostic tests.
| Item Type: | Article (Abstract) |
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| Additional Information: | 7963/115259 |
| Uncontrolled Keywords: | Dengue infection, metabolomics, gas chromatography-mass spectrometry (GC-MS), biomarker |
| Subjects: | Q Science > QP Physiology R Medicine > RA Public aspects of medicine > RA643 Communicable Diseases and Public Health |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine |
| Depositing User: | Dr Wan Fatein Nabeila Wan Omar |
| Date Deposited: | 15 Nov 2024 15:43 |
| Last Modified: | 15 Nov 2024 15:43 |
| URI: | http://irep.iium.edu.my/id/eprint/115259 |
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