Hyaluronidase involvement in Streptococcus pneumoniae biofilm activity

Ahmad Suaidan, Muhammad Abdullah and Mohd Amin, Nor Munirah and Enche Othman, Mohammad Izwan and Mohd Nayian, Nurul Azahana and Abu Mansor, Abu Zarrin and Ahmad Yusof @ Hanafi, Hanani (2024) Hyaluronidase involvement in Streptococcus pneumoniae biofilm activity. ASM Science Journal, 19. pp. 1-7. ISSN 1823-6782 E-ISSN 2682-8901

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Abstract

Streptococcus pneumoniae causes numerous severe infections and results in a significant number of mortality cases due to various virulence factors, including its ability to form a biofilm. The antimicrobial resistance and the challenges the host immune system faces in combating the biofilm make treatment against S. pneumoniae increasingly difficult. Consequently, investigations into pneumococcal biofilm are of paramount importance. The formation and enhancement of pneumococcal biofilm have been found to be influenced by various molecules, including hyaluronic acid. The degradation of hyaluronic acid by its specific enzyme, hyaluronidase, is believed to amplify the pathogenicity of pneumococci through increased colonisation. To delve deeper, the roles of hyaluronidase and hyaluronic acid in pneumococcal biofilm formation were investigated. The wild-type and Δhyl S. pneumoniae strains were cultivated in Brain Heart Infusion (BHI) media, both with and without the addition of hyaluronic acid, for six days. The formation of the biofilm at each incubation interval was identified using a crystal-violet (CV) microplate biofilm screening assay. These readings were analysed using the non-parametric Mann-Whitney test. The Δhyl mutant strain generally exhibited lower biofilm production during the initial four days but significantly surpassed the wild-type strain on days 5 and 6. No notable differences in biofilm measurements were observed between the two strains when cultured in media enriched with hyaluronic acid. Hyaluronidase appears to play a role in the biofilm cycle of S. pneumoniae, whereas additional hyaluronic acid in the culture medium seems to have no marked impact on biofilm activities. Further research is necessary to gain a clearer understanding of hyaluronidase’s function in S. pneumoniae biofilm activities.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Streptococcus pneumonia; biofilm; hyaluronidase; hyaluronic acid
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences
Depositing User:	Dr Hanani Ahmad Yusof @ Hanafi
Date Deposited:	26 Jul 2024 12:01
Last Modified:	26 Jul 2024 17:09
URI:	http://irep.iium.edu.my/id/eprint/113414

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