Assessment of antibacterial activity of fresh garlic juice extract against Vibrio spp. isolated from hybrid grouper

Razali, Muhamad Izzuan and Nawi, Mohd Firdaus and Mohd Idris, Shaharah and Abdullah, Azila and Ramly, Rimatulhana and Mansor, Nur Nazifah and Mohammad Ridzuan, Mohd Syafiq and Mustafa, Sufian (2024) Assessment of antibacterial activity of fresh garlic juice extract against Vibrio spp. isolated from hybrid grouper. Malaysian Fisheries Journal, 24 (Special Issue 2). pp. 34-44. ISSN 1511-7286

PDF (Journal) - Published Version Restricted to Registered users only Download (46MB) | Request a copy

Abstract

Antimicrobial resistance has gained worldwide attention. Investigations of the antimicrobial properties of plants and botanical substances, particularly garlic (Allium sativum) have attracted significant attention due to their promising abilities in this field. This study evaluated the antibacterial activity of fresh garlic juice extract against Vibrio spp. isolated from hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus). The antibacterial activity of different concentrations of garlic juice extract was evaluated using the Kirby-Bauer disc diffusion susceptibility test. The positive and negative controls were commercial antibiotic and ethanol, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract against Vibrio alginolyticus, V. vulnificus, and V. parahaemolyticus were determined using broth dilution. The Vibrio spp. isolates exhibited varying sensitivity levels to the garlic juice extract concentrations. The inhibitory zone diameter varied with the Vibrio species used but remained constant with regardless of the amount and concentration of fresh garlic juice extract used. V. vulnificus was the least sensitive to 100% fresh garlic juice extract, followed by V. parahaemolyticus and V. alginolyticus. The inhibition zone diameter increased together with the increased concentrations of garlic juice extract. The garlic juice extract MIC and MBC against Vibrio spp. were <1%. The results demonstrated that a small amount of fresh garlic juice extract (<1%) was sufficient to inhibit the bacterial growth. Thus, garlic juice extract was effective against the test bacteria and can be utilised to treat vibriosis in hybrid grouper.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Epinephelus spp., Garlic juice extract, Vibrio spp., antimicrobial activity
Subjects:	Q Science > QR Microbiology S Agriculture > SH Aquaculture. Fisheries. Angling S Agriculture > SH Aquaculture. Fisheries. Angling > SH151 Aquaculture - Fish Culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Marine Science Kulliyyah of Science > Institute of Oceanography and Maritime Studies
Depositing User:	Dr Mohd Firdaus Nawi
Date Deposited:	15 Jul 2024 08:39
Last Modified:	15 Jul 2024 15:51
URI:	http://irep.iium.edu.my/id/eprint/113141

Actions (login required)

View Item