DNA aptamers: the game-changers in diagnostics

Abdul Rashid, Nur Nadiah and Mohd Nasir, Mohd Hamzah and Hamzah, Nurasyikin and Abdul Hamid, Azzmer Azzar and Mohamed Suffian, Izzat Fahimuddin (2024) DNA aptamers: the game-changers in diagnostics. Revelation and Science, 14 (01). pp. 59-74. ISSN 2229-9645 E-ISSN 2229-9947

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Abstract

Rapid and efficient diseasediagnosis is crucialfor prompt clinical intervention.The current diagnosis methods require improvements, in terms of sensitivity, specificity,and cost.Aptamers, which are synthetic oligonucleotides, have gained popularityin the diagnostic biosensors’ development as theydetect analytes, viruses, and even cancerous cellswith high accuracy.They are selected through SELEX or in silico SELEX, leading to sequences thatbindwithspecific targetsand form aptamer-target complexes.Recently, DNA aptamers are preferredcompared to RNA aptamersin many studies, due to theirstability and ability to be chemically modified.The cost-effectivenessof aptamersenhancesresearch accessibility, facilitates large-scale production,andsupports the affordability ofdiagnostics, includingthe production of POCT kits.This paper discusses the structure fundamentaland motifsofDNA aptamerssuch as hairpin,G-quadruplex,and pseudoknot.The aptamer-based diagnostic platformsin detectingvarious diseasesand the challenges of DNA aptamers are highlightedin this review

Item Type:	Article (Journal)
Uncontrolled Keywords:	aptamer, biosensor, DNA hairpin, lateral flow assay
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Mohd Hamzah Mohd Nasir
Date Deposited:	03 Jul 2024 10:58
Last Modified:	03 Jul 2024 10:58
URI:	http://irep.iium.edu.my/id/eprint/112945

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