Pluchea indica less leaves extract as a root canal irrigant against Enterococcus faecalis colonies: ex vivo study

Fibryanto, Eko and Tio, Abdil and Gunawan, Juanita Amaludin and Hidayat, Adi and Mohd Noh, Nur Zety (2024) Pluchea indica less leaves extract as a root canal irrigant against Enterococcus faecalis colonies: ex vivo study. In: Quality Improvement in Dental and Medical Knowledge, Research, Skills and Ethics Facing Global Challenges. Proceedings of the International Conference on Technology of Dental and Medical Sciences (ICTDMS 2022) (1). CRC Press, Taylor & Francis Group, London, United Kingdom, pp. 116-123. ISBN 978-1-032-51441-3

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Abstract

Enterococcus faecalis is the most prevalent bacteria in root canals. One of the herbal solutions that can be used as antibacterial irrigation is Pluchea indica Less leaves. This study aims to analyze the antibacterial effects of P. indica L. leaves extract with concentrations of 12.5%, 25%, and 50% as a root canal irrigant against E. faecalis. Twenty-four sound human mandibular first premolars were endodontically prepared and inoculated with E. faecalis, then randomly allocated into six groups. Test groups were irrigated either with 12.5%, 25%, or 50% concentrations of the extract. The control groups were irrigated with either 1.25% and 5.25% NaOCl, or PBS. The inhibition test was conducted using the agar diffusion method and the number of bacterial colonies remaining in the root canal after irrigation was measured with the plate count method. The differences in antibacterial activity and the number of colonies between groups were analyzed using a one-way analysis of variants (ANOVA) test and post-hoc Tukey test (p<0.05). There were significant differences in the number of E. faecalis colonies between groups (p<0.05). The E. faecalis colony was found to be absent in the 1.25% and 5.25% NaOCl groups. The number of E. faecalis colonies in 12.5%, 25%, and 50% concentrations of the P. indica L. leaves extract was 13.2 ± 1.6 × 105CFU/mL, 9.90 ± 1.32 × 105CFU/mL, and 3.1 ± 2.08 × 10 CFU/mL, respectively. The 50% concentration of the P. indica L. leaves extract showed the best results in inhibiting growth and reducing the amount of E. faecalis colonies in the root canal.

Item Type:	Book Chapter
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Restorative Dentistry Kulliyyah of Dentistry
Depositing User:	NUR ZETY MOHD NOH
Date Deposited:	02 May 2024 16:19
Last Modified:	02 May 2024 17:22
URI:	http://irep.iium.edu.my/id/eprint/112013

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