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The effect of supplementation of Lactococcus lactis strain as probiotic on the growth and survival of Litopenaeus vannamei

Baharuddin, Nur Alya Syafiqah and Wan Abdul Ghani, Wan Alia and Che Othman, Siti Fairuz and Nawi, Mohd Firdaus and Tengku Abdul Hamid, Tengku Haziyamin (2024) The effect of supplementation of Lactococcus lactis strain as probiotic on the growth and survival of Litopenaeus vannamei. Notulae Scientia Biologicae, 16 (1). pp. 1-14. ISSN 2067-3205 E-ISSN 2067-3264

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Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) is a bacterial disease in shrimps caused by pathogen Vibrio parahaemolyticus. It has culminated in huge loss in global shrimp production due to mass mortality. A probiotic strain Lactococcus lactis strain FA1 was recently isolated from shark intestine, showing inhibition towards the growth ofthe pathogen. Due to inhibitory potential, the effect of probiotics strain on growth performance of shrimpinfected with V. parahaemolyticus was evaluated. The probiotic strain was incorporated into feed for juvenile shrimps Litopanaeus vannamei for 3 weeks before which they were then challenged with pathogen Vibro parahaemolyticus. The study compares 4 shrimp groups: Control (Without any treatment); Group A (Probiotic treated, uninfected); Group B (Probiotic treated, infected) and Group C (No probiotic, infected). The survival and growth performance (weight and lengthgain) of shrimps were evaluated in the following 30 days. Statistical analyses (ANOVA; Post Hoc Tukey) were used to compare between shrimp groups. In general, infected shrimp demonstrated some of the key symptoms of AHPND (pale or white hepatopancreas), transparent body and erratic swimming behaviour. The supplementation of probiotics resulted in an improved survivability (65±1%) compared to infected shrimp (45±1%). The probiotic treated shrimp group showed to have better % body weight gain, in which weight gain between group B (treated/infected) and group C (untreated/infected) were significantly different (at P=0.046); and so, to group A (treated/uninfected) when compared with group C (P=0.047). There is however no significant difference in % length gain between the groups. Meanwhile, some of the infected shrimps were able to show recovery from the infection. Pre-infection probiotic treatment showed better performance compared to post-infection probiotic treatment. This indicated that the strain Lactococcus lactis is highly suitable for use as the future probiotic in shrimp aquaculture.

Item Type: Article (Journal)
Uncontrolled Keywords: acute hepatopancreatic necrosis disease; early mortality syndromes; Lactococcus lactis; Litopenaeus vannamei; shrimp probiotics
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science
Kulliyyah of Science > Department of Biotechnology
Kulliyyah of Science > Department of Marine Science
Depositing User: Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited: 05 Apr 2024 13:46
Last Modified: 26 Jun 2024 11:47
URI: http://irep.iium.edu.my/id/eprint/111327

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