Ullah, Saeed and Huyop, Fahrul Zaman and Huda, Nurul and Ab Wahab, Roswanira and Abdul Hamid, Azzmer Azzar and Mohamad, Mohd Azrul Naim and Ahmad, Hajar Fauzan and Mohd Shariff, Amir Husni and Mohd Nasir, Mohd Hamzah (2024) Green honey of Banggi Island: a preliminary anti-diabetic study on zebrafish model. Heliyon, 10 (4). pp. 1-8. E-ISSN 2405-8440
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Abstract
Zebrafish is a developing vertebrate model with several advantages, including its small size, and high experimental efficiency. Malaysia exhibit one of the highest diabetes rates in the Western Pacific and incurring an annual cost of 600 million US dollars. The objective of the study is to determine the antidiabetic properties of green honey (GH) using a zebrafish model. Adult zebrafish, aged 3–4 months, were subjected to overfeeding and treated with streptozotocin (STZ) through intraperitoneal injection (IP) on days 7 and 9. The study assessed the oral sucrose tolerance test (OSTT) and the anti-diabetic effects of green honey. The evaluation was conducted at three time points: 30, 60, and 120 min after treatment and sucrose administration. The study utilised a model with a sample size of 5. The study was performed in six groups. These groups are (1) Normal control (non-diabetic, no intervention), (2) Normal control + GH (non-diabetic, supplemented with GH 3 μl), (3) DM control (diabetic, no intervention), (4) DM Gp1 (diabetic, 3 μL GH), (5) DM Gp2 (diabetic, 6 μ L GH), (6) DM Acarbose (diabetic, treated with acarbose). Fasting blood glucose levels for non-diabetic (non-DM) and diabetic (DM) groups were evaluated before and after the 10 days of diabetic induction. DM groups (excess of food and two injections of STZ) have caused a significant increment in the fasting blood glucose to 11.55 mmol/l (p < 0.0001). Both GH treatments effectively decreased postprandial blood glucose levels and the area under the curve in the oral glucose tolerance test (OSTT). Based on these results, it is concluded that green honey could play a role in hyperglycemia management and show potential as a natural alternative to conventional diabetes therapy. The underlying mechanisms need to be clarified, and their potential use in human diabetes therapy needs to be investigated.
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | Green honey Zebrafish Antidiabetic STZ Acarbose |
| Subjects: | Q Science > Q Science (General) |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology |
| Depositing User: | Dr Mohd Hamzah Mohd Nasir |
| Date Deposited: | 20 Feb 2024 09:00 |
| Last Modified: | 06 Mar 2024 14:28 |
| URI: | http://irep.iium.edu.my/id/eprint/110901 |
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