Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos

Warsi, Warsi and Jaswir, Irwandi and Ahmed, Qamar Uddin and Mahfudh, Nurkhasanah and Mohd. Nawi, Mohamed Sufian and Rohman, Abdul and Khatib, Alfi (2024) Morphological, teratogenic and behavioral evaluations of Gelidium spinosum methanol extract on zebrafish embryos. Pharmacia, 71. pp. 1-10. ISSN 0428-0296 E-ISSN 2603-557X

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Abstract

Gelidium spinosum is an edible red seaweed from the family Gelidiaceae with possibility to be developed. The potential medical benefits of G. spinosum have been established, yet adequate empirical research on its toxicity is still lacking. Hence, the present work was aimed to examine the toxicity of G. spinosum methanol extract (GsME) on zebrafish (Danio rerio) embryos and to identify the phytoconstituents using gas chromatography-mass spectrometry (GC-MS) analysis. Results of this study showed that GsME induced morphological defects in zebrafish embryos, including reduction in eye size and body length. Moreover, yolk sac size and mortality were increased in zebrafish embryos exposed to GsME in a dose-dependent manner. GsME at high concentrations triggered teratogenic effects in zebrafish embryos such as decrease in heartbeat/minute, lack of pigmentation, lack of somite, structural deformity, pericardial oedema, and yolk oedema. The LC50 and EC50 of GsME were < 100 mg/L, which classified as a harmful category. The teratogenic index of GsME was found to be > 1, indicating its teratogenic attribute. Additionally, GsME exerted behavioral effects i.e. significantly lower total distance of movement and slower swimming speed of zebrafish embryos. GC-MS analysis of GsME was confirmed the presence of amino acid, phenolics, carboxylic acids, reducing sugars, saturated fatty acids and brominated saturated fatty acid in the extract. It suggesting that compounds of 13-bromotetradecanoic acid, palmitic acid, and stearic acid containing in GsME were contributed to the toxic effects.

Item Type:	Article (Journal)
Additional Information:	5784/110716
Uncontrolled Keywords:	Behavior, Gelidium spinosum, GC-MS, Red seaweed, Toxicity
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Ms Yasmin Mastura Mustafa Byrnes
Date Deposited:	05 Feb 2024 11:55
Last Modified:	29 Jul 2024 17:36
URI:	http://irep.iium.edu.my/id/eprint/110716

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