The cytotoxic activity of marine sponge-derived fungus aspergillus nomius NC06 against HT29 colon cancer cells

Artasasta, Muh. Ade and Djamaan, Akmal and Yanwirasti, Yanwirasti and Bakhtiar, M. Taher and Djamaludin, Heder and Siswanto, Siswanto and Handayani, Dian (2024) The cytotoxic activity of marine sponge-derived fungus aspergillus nomius NC06 against HT29 colon cancer cells. Journal of Tropical Life Science, 14 (1). pp. 39-44. ISSN 2087-5517 E-ISSN 2527-4376

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Abstract

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities. This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Aspergillus nomius, Cytotoxic activity, HT29 colon cancer cell
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Muhammad Taher
Date Deposited:	31 Jan 2024 14:24
Last Modified:	29 Jul 2024 16:57
URI:	http://irep.iium.edu.my/id/eprint/110617

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