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Genome size determination of Ficus deltoidea using flow cytometry method

Zailani, Siti Sarah and Fikri, Muhammad Irfan and Midin, Mohd Razik (2021) Genome size determination of Ficus deltoidea using flow cytometry method. In: Recent Advances in Ficus Research. Noor Publishing, pp. 77-83. ISBN 978-620-3-86004-7

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Ficus deltoidea is a large shrub native to Southeast Asia. locally, it is known as 'Mas cotek'. It is famous for its medicinal value and health benefits. As for now, genome size data of F. deltoidea is not available yet. This study aims to optimise nuclei preparation method of flow cytometry (FCM) measurement as well as determine the nuclear genome size of F. deltoidea. Several steps involved in this study: (1) isolation of nuclei using two different techniques (manual chopping and Medi-machine) and two different lysis buffers (Otto and LBO1), (2) nuclear DNA staining using propidium iodide (PI) dye and (3) measurement of fluorescent intensity of stained nuclei. DNA histograms generated from different methods and lysis buffers were compared. This study revealed that the best DNA histogram was obtained by using manual chopping technique supplemented with Otto buffer. Using FCM with Glycine max cv. Polanka (2C=2.5pg) as an external reference standard, the genome size of F. deltoidea was found to be 2.88 pg. The genome size data can be utilised in future studies of ecology, evolution, taxonomy as well as genome sequencing.

Item Type: Book Chapter
Uncontrolled Keywords: Ficus deltoidea, flow cytometry, genome size
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH426 Genetics
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science
Kulliyyah of Science > Department of Plant Science
Depositing User: Dr Mohd Razik Midin
Date Deposited: 31 Jan 2024 16:05
Last Modified: 31 Jan 2024 16:14
URI: http://irep.iium.edu.my/id/eprint/110525

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