Analysis of antioxidant and anticancer properties in Methanolic extract of piper Sarmentosum Roxb. against MCF-7 Cells

Ibrahim, Maizatul Akma and Ghazali, Nor Farahiyah and Zakaria, Nor Hafizah and Abdul Majid, Fadzilah Adibah (2023) Analysis of antioxidant and anticancer properties in Methanolic extract of piper Sarmentosum Roxb. against MCF-7 Cells. Revelation and Science, 1 (Special Issue 1). pp. 20-28. ISSN 2229-9645 E-ISSN 2229-9947

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Abstract

Cancer is by far one of the most worrying health issues that continue to be the major killing diseases worldwide, accounting for more than six million deaths. In the confrontation of advances in modern medicine such as surgery, chemotherapy, radiotherapy, and hormone therapy, cancer disease persists as a worldwide problem. In recent years, there has been a focused effort to explore new alternatives, with a particular emphasis on identifying medicinal herbs from local natural resources. Approximately, 60% of cancer drugs are derived from natural sources, due to their fewer side effects. Piper sarmentosum Roxb. which is locally known as ‘kaduk’, is a natural medicinal plant that has been used traditionally to treat headache, arthritis, menstrual pain, cough, and eczema. It is proven to have various biological properties such as anti-inflammatory, antioxidant, antimalarial, antiplasmodial, anti-diabetic, antifungal, and anti-carcinogenic. In this study, the phenolic content of P. sarmentosum was quantified using Folin-Ciocalteu method. Next, the antioxidant activity was determined using a DPPH scavenging assay. MTS assay was conducted to analyse the effect of the extract on human breast cancer cells, MCF-7. The results showed that the P. sarmentosum extract contained a high TPC which was 89.22 mg GAE/g. The extract also exhibited a good antioxidant activity with an EC50 value of 96.98 ± 2.29 μg/mL. The in vitro cytotoxicity test on MCF-7 cell proliferation showed the extracts’ IC50 at 24.63 ± 0.23 μg/mL. In conclusion, methanolic extract of P. sarmentosum contained a high phenolic content and is a potent antioxidant capacity as well as anticancer activity against the tested breast cancer cell.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Breast cancer, medicinal plant, Piper sarmentosum, phenolic content, radical scavenging activity
Subjects:	B Philosophy. Psychology. Religion > B Philosophy (General) BPC Science and Technology in Islam Q Science > Q Science (General) Q Science > Q Science (General) > Q128 Islam and Science Q Science > QK Botany Q Science > QR Microbiology R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RZ Other systems of medicine S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Plant Science Kulliyyah of Science
Depositing User:	Dr Maizatul Ibrahim
Date Deposited:	18 Jan 2024 14:35
Last Modified:	18 Jan 2024 14:36
URI:	http://irep.iium.edu.my/id/eprint/110237

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