In vitro and in silico screening analysis of artabotrys sumatranus leaf and twig extracts for α-glucosidase inhibition activity and its relationship with antioxidant activity

Rosa, Dela and Elya, Berna and Hanafi, Muahamad and Khatib, Alfi and Surya, Muhammad Imam (2023) In vitro and in silico screening analysis of artabotrys sumatranus leaf and twig extracts for α-glucosidase inhibition activity and its relationship with antioxidant activity. Scientia Pharmaceutica, 91 (1). pp. 1-18. ISSN 0036-8709 E-ISSN 2218-0532

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Abstract

Artabotrys sumatranus is one of the Artabotrys species, which lives in Sumatera, Java, and Borneo in Indonesia. No research has been found related to its activity. The objective of this study was to explore the potential of A. sumatranus leaf and twig extracts as the source of an anti-diabetic agent through the α-glucosidase inhibition mechanism, as well as the relationship between the antioxidant and the α-glucosidase inhibition activities in these extracts. Ethanol extracts from leaf and twig A. sumatranus were subjected to several assays: total phenolic content, total flavonoid content, antioxidant activity using DPPH (2,2-diphenyl-1-picrylhydrazyl), radical scavenging activity, and FRAP (ferric reducing antioxidant power) analysis, as well as α-glucosidase inhibition. Later, GC-MS (gas chromatography-mass spectrometer) and LC-MS/MS (liquid chromatography-mass spectrometer/mass spectrometer) analysis were conducted to identify the compounds inside the extracts. The identified compounds were tested for potential α-glucosidase inhibition activity using a molecular docking simulation. As a result, the A. sumatranus leaf extract showed more potential than the twig extract as α-glucosidase inhibitor and antioxidant agent. In addition, from the comparison between the measured quantities, it can be deduced that most of the α-glucosidase active compounds in the A. sumatranus are also antioxidant agents. Several active compounds with a high affinity to α-glucosidase inhibition were identified using the molecular docking simulation. It was concluded that A. sumatranus twig and leaf extracts seem to be potential sources of α-glucosidase inhibitors.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Artabotrys sumatranus; antidiabetic; antioxidant; leaf extract; twig extract
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	07 Dec 2023 12:26
Last Modified:	11 Dec 2023 16:19
URI:	http://irep.iium.edu.my/id/eprint/108665

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