Optimisation of lignocaine-adrenaline nanoemulsion using central composite design for gingival retraction cord application

Azman, Min Mardhiyyah and Haris @ Harith, Muhammad Salahuddin and Wan Abd Manan, Wan Nor Hayati (2023) Optimisation of lignocaine-adrenaline nanoemulsion using central composite design for gingival retraction cord application. In: IIUM National Oral Health Research Initiatives (NOHRI) Conference, 20-21 October 2023, Kuantan, Pahang. (Unpublished)

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Abstract

Introduction: A commonly employed technique for gingival displacement involves the insertion of retraction cord into the gingival crevice, which leads to bleeding and pain when used for extended periods especially in a complex restorative dental treatment. The gingival retraction cord is soaked in the ferric sulphate to control bleeding, but it can cause tooth staining and recurrent bleeding post-cord removal. Local anaesthesia with lignocaine is administered for pain relief, yet it may induce heightened dental anxiety (DA), contributing to patient fear and stress during the dental procedures. Therefore, lignocaine-adrenaline combination is valued for its pain-relieving and hemostatic properties. Aims: Thus, this research aims to optimise the lignocaine-adrenaline nanoemulsion (LANE) using central composite design (CCD) for improving gingival retraction cord application. Methods: A total of 18 LANE formulations obtained from CCD were prepared by spontaneous emulsi;ication method in which the particle size, zeta potential, pH, polydispersity index (PdI), and stability at 25 °C for 72 hours were evaluated. Based on the analysed results, the optimised LANE was validated in triplicate. Results: CCD suggested an optimised LANE with 4% (w/w) Triacetin (oil phase), 12% (w/w) Tween 80 (surfactant), and 9.71% (w/w) PEG 400 (co-surfactant). The validated optimised formulation exhibited 25.30 ± 1.12 nm particle size, -18.80 mV ± 0.87 zeta potential, 7.58 ± 0.05 pH, and 0.43 ± 0.01 PdI. The LANE formulation was stable and observed to be clear, with no drug precipitation and phase separation. Conclusion: The optimised LANE formulation has been achieved through CCD. The optimised LANE will be formulated further as a nanogel-based gingival retraction cord that holds the potential to alleviate pain and DA. This development could transform dental practices, fostering enhanced patient experiences and successful restorative dental treatments.

Item Type:	Conference or Workshop Item (Poster)
Uncontrolled Keywords:	Lignocaine, Adrenaline, Nanoemulsion, Central Composite Design, Gingival Retraction Cord
Subjects:	R Medicine > RK Dentistry R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Prosthodontics Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Dentistry Kulliyyah of Pharmacy
Depositing User:	Dr. Muhammad Salahuddin Haris
Date Deposited:	25 Oct 2023 12:38
Last Modified:	25 Oct 2023 12:38
URI:	http://irep.iium.edu.my/id/eprint/107659

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