Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S mitochondrial rRNA gene sequences

Kamarudin, Kamarul Rahim and Hashim, Ridzwan and Usup, Gires (2010) Phylogeny of sea cucumber (Echinodermata: Holothuroidea) as inferred from 16S mitochondrial rRNA gene sequences. Sains Malaysiana, 39 (2). pp. 209-218. ISSN 0126-6039

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Abstract

This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) showed the presence of five main genera of sea cucumbers: Molpadia from order Molpadiida and four genera of order Aspidochirotida namely Holothuria, Stichopus, Bohadschia and Actinopyga. All of the 17 species obtained from Malaysia distributed among the main genera except within Interestingly, Holothuria excellens was out of Holothuria group causing Holothuria to be paraphyletic. High bootstrap value and consistent clustering made Molpadia, Stichopus, Bohadschia and Actinopyga monophyletic. The relationship of with the other genera was unclarified and Stichopus was sister to Molpadia. The latter finding caused the resolution at order level unclear. The pairwise genetic distance calculated using Kimura 2-parameter model further supported and verified findings from the phylogenetic trees. Further studies with more samples and different mitochondrial DNA genes need to be done to get a better view and verification on the molecular phylogeny of sea cucumbers.

Item Type:	Article (Journal)
Additional Information:	4689/10708
Uncontrolled Keywords:	Phylogenetic relationship; partial sequences of 16S mitochondrial ribosomal RNA gene; sea cucumbers
Subjects:	R Medicine > RS Pharmacy and materia medica S Agriculture > SH Aquaculture. Fisheries. Angling
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Science > Department of Biomedical Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Ms Rosyidah Taju Rahim
Date Deposited:	20 Dec 2011 08:25
Last Modified:	20 Dec 2011 08:25
URI:	http://irep.iium.edu.my/id/eprint/10708

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