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Prospect of antagonising Vibrio disease in shrimp using quorum sensing proteins: I in silico studies base on docking interaction

Zainudin, Ahmad ‘Ammar and Abdul Hamid, Azzmer Azzar and Tengku Abdul Hamid, Tengku Haziyamin (2023) Prospect of antagonising Vibrio disease in shrimp using quorum sensing proteins: I in silico studies base on docking interaction. In: 5th AFOB Malaysia Chapter International Symposium 2023, 18th - 19th September 2023, Online. (Unpublished)

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V. parahaemolyticus is a disease-causing bacterium responsible for acute hepatopancreatic necrosis disease (AHPND); or referred to as Early Mortality Syndrome (EMS) disease in shrimp aquaculture. Infected shrimp shows sign of pale or shrunken hepatopancreas that disrupts the digestive tract. Quorum sensing mechanism is one of a common method of communication used by bacteria in survival or adapting to the changing environment. Vibrio parahaemolyticus may use quorum sensing mechanism during shrimp colonisation; or in eliciting its pathogenicity. In a quorum sensing pathway, an autoinducer is initially activated which subsequently triggers other virulent gene expression. Studies have shown that there are 3 quorum sensing proteins leading to virulent gene expression: namely LuxO, AphA and OpaR. Thus, the activation or suppression of any of these proteins could antagonise the pathogen and render them harmless to the host. It is anticipated that nisin-like bacteriocin (a short peptide) from Lactococcus lactis was found to be able to antagonise the V. parahaemolyticus by disrupting or interfering with quorum sensing mechanism in bacteria. To test on this, LuxO, AphA and OpaR were computationally docked with bacteriocin from L. lactis. Having constructed the structures of these 3 three proteins via homology modelling, each protein was then docked with bacteriocin. Docking interaction with bacteriocin showed that LuxO bind with the lowest binding energy (- 214.72 kcal/mol), followed by OpaR (-208.39 kcal/mol) and AphA (-195.29 kcal/mol). In addition to polar interaction, hydrophobic interaction plays key role in the binding of bacteriocin with LuxO, but not with AphA and OpaR. Thus, interaction of LuxO protein with bacteriocin will set series of gene expression necessary to make bacteria harmless. Thus, quorum sensing protein LuxO can be considered as a potential target in future works on antagonising V. parahaemolyticus to address EMS disease.

Item Type: Conference or Workshop Item (Keynote)
Uncontrolled Keywords: V. parahaemolyticus, quorum sensing, virulent gene expression, nisin-like bacteriocin
Subjects: Q Science > Q Science (General)
S Agriculture > S Agriculture (General) > S494.5.S86 Sustainable agriculture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Kulliyyah of Science
Depositing User: Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited: 25 Sep 2023 12:57
Last Modified: 25 Sep 2023 12:57
URI: http://irep.iium.edu.my/id/eprint/107034

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