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High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector

Helaluddin , Abul Bashar Mohammed and Saeed , Mohammad and Khan, Umeed Ali (2010) High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector. In: 3rd International Conference on Science & Technology : Applications in Industry and Education (ICSTIE 2010), 16-17 Dec 2010, Penang.

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Abstract

A reverse phase high performance liquid chromatographic method for the detection of the neurotransmitters namely serotonin, tryptophan and dopamine in human blood samples has been developed. Spiked standard samples were analysed using a fluorescence detector after chromatographic separation. For the analytes a working range of 2.5 ng/mL to 25 ng/mL were found suitable for the application. For chromatographic separation C18 type of column showed the better separation results and was used for the study. Mobile phase was optimised to 97% phosphate buffer and ion pairing reagent (pH adjusted to 5.5) together with 3% acetonitrile. Sample preparation step was found to be more effective with protein precipitation method than the solvent extraction method. Two protein precipitation methods were compared and found that perchloric acid method was more suitable than that of acetonitrile method. Intra and inter day precision of the analytes were determined and the coefficient of variation (CV) were less 15% which is within the acceptable limit of standard guidelines. Specificity of the analytes was established by comparing the standard peaks with the blank plasma chromatogram. The analysis results showed that the method is specific, accurate and reliable for the quantification of the analytes in human samples.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4478/10676
Uncontrolled Keywords: Neurotransmitters, HPLC, Fluorescence detector
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Psychiatry
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr. A. B. M. Helal Uddin
Date Deposited: 17 Oct 2013 11:16
Last Modified: 17 Oct 2013 12:19
URI: http://irep.iium.edu.my/id/eprint/10676

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