Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry

Mahdi, Harith Jameel and Budi Muljono, Retno Andayani and -, Ishak (2010) Metabolic fingerprinting of three Malaysian ginger (Zingiber officinale Roscoe) using gas chromatography-mass spectromertry. American Journal of Applied Science, 7 (1). pp. 17-23. ISSN 1546-9239

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Abstract

Problem statement: There is a chemical variation among different ginger cultivars detected by previous studies including the volatile and non-volatile oleo-resins. In this study we try to determine whether these variations are related to a generic factor rather than environmental or intrinsic factors. Approach: A comprehensive metabolic fingerprinting from the leaves of three micro-propagated ginger cultivars Bukit Tinggi, Tanjung Sepat and Sabah was performed using a Gas Chromatography-Mass Spectrometry (GC-MS). Constituents of the ginger leaves were first extracted and then fractionated into methanolic and chloroform. The samples were subsequently methoximated and silylated prior to GC-MS analysis. Results: By applying this technique, we detected more than 300 compounds (polar and non-polar) in total originated from each ginger cultivars. Based on the GC-MS fragmentation, three different classes of metabolites were detected from the ginger cultivars, namely amino acids, carbohydrates and organic acids. A qualitative variation on the type of ginger metabolites was observed, albeit no marked different found in the level of the metabolites. Conclusion: Apparently the chemical variations among the three ginger cultivars were due to genetic effects since almost all other environmental and intrinsic factors were eliminated.

Item Type:	Article (Journal)
Additional Information:	4790/10627
Uncontrolled Keywords:	Ginger, metabolic fingerprinting, gas chromatography-mass spectrometry, derivatisation
Subjects:	Q Science > Q Science (General) R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr Retno Andayani Budi Muljono
Date Deposited:	19 Dec 2011 15:27
Last Modified:	13 Jan 2012 17:21
URI:	http://irep.iium.edu.my/id/eprint/10627

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