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Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications

Haque, Tanjum Ara and Urmi, Umme Laila and Md Khademul Islam, Abul Bashar Mir and Ara, Bayasrin and Nahar, Shamsun and Md Mosaddek, Abu Syed and Lugova, Halyna and Kumar, Santosh and Jahan, Dilshad and A. Rahman, Nor Azlina and Haque, Mainul and Islam, Salequl and Godman, Brian (2022) Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications. Journal of Applied Pharmaceutical Science, 12 (4). pp. 185-198. ISSN 2231-3354

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Background: Plasmid-mediated quinolone-resistant (PMQR) genes and mutations within the quinolone resistance determining regions (QRDRs) resulted in the advent of quinolone-resistant pathogenic microbes. This research was designed to assess the roles of three PMQR genes, qnrA, qnrB, and qnrS, and any mutation in the gyrA gene in the QRDR as a process of quinolone/fluoroquinolone resistance to urinary tract infection (UTI) bacteria in Bangladesh to guide future management of UTIs. Methods: Pathogens from UTIs were isolated and identified, and their phenotype antibiotic susceptibilities were tested for lomefloxacin, ofloxacin, ciprofloxacin, and nalidixic acid. Polymerase chain reaction (PCR) detected the qnrA, qnrB, and qnrS genes. PCR and sequencing were performed to evaluate any mutation within the QRDRs of the gyrA gene. Results: Of 100 UTI bacteria, phenotypic resistance was observed in 95.0%, 89.0%, 83.0%, and 71.0% against lomefloxacin, nalidixic acid, ofloxacin, and ciprofloxacin, respectively. PMQR genes qnrS, qnrA, and qnrB genes were found in 54.0%, 1.0%, and 4.0% of isolates, respectively. Sequencing the gyrA gene revealed single mutation (Ser-83 to Leu) and double mutations (Ser-83 to Leu and Asp-87 to Asn). PMQR genes showed a statistically nonsignificant association with phenotypic resistance. Conclusions: This study confirms the presence of QRDR mutations that were independent of PMQR genes. Consequently, high resistance against quinolones among uropathogens is evident, and their future use needs to be moderated.

Item Type: Article (Journal)
Uncontrolled Keywords: Quinolone, PMQR, qnr, QRDR, UTI, Bangladesh.
Subjects: R Medicine > R Medicine (General)
R Medicine > RZ Other systems of medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Physical Rehabilitation Sciences
Depositing User: Dr. Nor Azlina A Rahman
Date Deposited: 17 Aug 2023 16:52
Last Modified: 17 Aug 2023 16:59
URI: http://irep.iium.edu.my/id/eprint/106030

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