Glyphosate suppresses the ability of PON1 to Hydrolyse Oxidized-LDL in the exposed farm workers in Pahang, Malaysia

Ishaka, Aminu and Abdullah, Nor Zamzila and Samsuddin, Niza and Mohd Rus, Razman and Mohamed, Rozilah @ Abdul Hadi (2023) Glyphosate suppresses the ability of PON1 to Hydrolyse Oxidized-LDL in the exposed farm workers in Pahang, Malaysia. IIUM Medical Journal Malaysia (IMJM), 22 (3). pp. 105-113. ISSN 1823-4631 E-ISSN 2735-2285

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Abstract

INTRODUCTION: Paraoxonase 1 (PON1) is a high-density lipoprotein (HDL)-associated enzyme which is known to hydrolyse most pesticides including organophosphates (OPs) and prevent atherosclerosis by inhibiting oxidative modification of low-density lipoprotein (LDL). Glyphosate is one of the common organophosphate pesticides used in agriculture in many developing countries including Malaysia. The aim of this study to is to assess the PON1 ability to hydrolyse oxidized LDL in glyphosate exposed farm workers. MATERIALS AND METHODS: In this cross-sectional comparative study, a total of 103 subjects (53 Glyphosate-exposed and 50 non-exposed) were recruited. Fasting serum samples were analysed for PON1 activities towards substrates paraoxon, phenylacetate, and diazoxon, as well as for lipid profiles and oxidized-LDL (ox-LDL). RESULTS: The results showed lower basal paraoxonase activity [156.96 (58.87) vs 177.06 (66.78)], arylesterase activity [90.06 (17.14) vs 96.92 (23.87)] and diazoxonase activity [850.93 (206.75) vs 990.48 (248.73)] in glyphosate-exposed compared to non-exposed, however, only diazoxonase activity was statistically significant (p<0.05). PON1 activity is not significantly different with different length of period of exposure except for arylesterase. There was also significantly higher (p<0.05) ox-LDL in the exposed group but no significant differences in lipid profiles (p>0.05) between the two groups. The PON1 to ox-LDL ratio which probably reflects the ability of PON1 to hydrolyse ox-LDL were also significantly lower (p<0.05) among the glyphosate-exposed group. CONCLUSION: The results suggested that the decreased PON1 activity in glyphosate-exposed individuals could predispose them to the development of atherosclerosis and coronary artery disease through decreased PON1 ability to hydrolyse ox-LDL.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Glyphosate exposure, PON1 activity, lipid profile, ox-LDL, farm workers.
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General) S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Anaesthesiology & Intensive Care Kulliyyah of Medicine > Department of Community Medicine (Effective: 1st January 2011) Kulliyyah of Medicine > Department of Pathology & Lab Medicine
Depositing User:	Ms Nuur Farhana Zulkefli
Date Deposited:	03 Aug 2023 14:35
Last Modified:	03 Aug 2023 14:35
URI:	http://irep.iium.edu.my/id/eprint/105801

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