Development of ultraviolet spectroscopic method for the estimation of metronidazole nanoparticles for periodontal disease treatment

Mohd Zayi, Nora Azirah and Mohamed Halim, Muhammad Lutfi and Harun @ Ismail, Ahmad Fahmi and Mohamad, Mohd Yusof (2023) Development of ultraviolet spectroscopic method for the estimation of metronidazole nanoparticles for periodontal disease treatment. Malaysian Journal of Chemistry, 25 (3). pp. 354-367. ISSN 1511-2292 E-ISSN 2550-1658

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Abstract

In periodontitis treatment, metronidazole (MT) is applied topically to reduce systemic side effects and reach the target site. treatment of periodontitis via sustained MT release. The UVspectrophotometer analysis was developed and validated to quantify the encapsulated MT according to ICH Q2 (R1) guidelines, which include parameters such as specificity, linearity, accuracy, precision (in the form of repeatability), the limit of detection (LOD) as well as the limit of quantification (LOQ), range, robustness, and ruggedness. Metronidazole nanoparticles (MTNP) were fabricated at different concentrations (0.15–0.60 mg/mL) using ionic gelation. The encapsulated MT was examined using a UV spectrophotometer, a Nano Zetasizer, Fourier transform infrared spectroscopy (FTIR), and a field emission scanning electron microscope (FESEM). The maximum wavelength (max) was discovered to be 320 nm, and it obeyed Beer's law with a linear relationship (R2 = 0.999) in the range of 2-12 µg/mL. The parameters analyzed met ICH Q2 (R1) standards. MT-NP had a spherical structure and absorption band similar to chitosan empty (CS), with a size of 308.0 ± 9.18 nm, a polydispersity index of 0.374 ± 0.37, 46.6± 0.23, and an encapsulation efficiency of 87.95 ± 0.07. These findings suggest that UV-visible can be a useful tool for the estimation of MT nanoparticles and MT-NP as promising local antibacterial agents to treat periodontitis.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Metronidazole nanoparticle; UV/Vis spectroscopy; local drug delivery; periodontal disease
Subjects:	Q Science > QD Chemistry R Medicine > R Medicine (General) R Medicine > RK Dentistry > RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Physical Rehabilitation Sciences Kulliyyah of Allied Health Sciences
Depositing User:	Dr Mohd Yusof Mohamad
Date Deposited:	17 Jul 2023 08:48
Last Modified:	17 Jul 2023 08:50
URI:	http://irep.iium.edu.my/id/eprint/105536

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