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On-site sequencing of SARS-COV-2 genomes identify two variant of concern clusters in Pahang, Malaysia

Zainulabid, Ummu Afeera and Wan Ramli, Wan Nurliyana and Haroon, Raihanah and Aslam, Ayesha and Hussain, Mushtaq and Ahmad, Hajar Fauzan (2023) On-site sequencing of SARS-COV-2 genomes identify two variant of concern clusters in Pahang, Malaysia. Current Science and Technology (CST), 2 (2). pp. 1-7. E-ISSN 2785-8804

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Timely variant identification is required in respond to effective public health measures. It has been demonstrated that comprehending genetic epidemiology can be aided by a combination of public health expertise and through viral genomic sequencing using Oxford Nanopore Technologies (ONT). The purpose of this study is to determine the utility of using Oxford Nanopore Sequencing to elucidate the genetic epidemiology in two significant clusters in Pahang, Malaysia. The combined oropharyngeal and nasopharyngeal swabs of clinical specimens from two significant clusters in Pahang, Malaysia, were retrieved for long-read sequencing. Results: We identify Beta and Delta variants as 2 variants of concern from our analysis. We found that the B.1.351 (ß) and B.1.617 (D) variants were responsible for the Taman Tanah Putih Baru and Pasar Kemunting clusters, respectively. In conclusion, ONT long-read sequencing tools offer a practical solution with a number of advantages. ONT devices are compact, affordable, and need little technical hands-on or laboratory equipment to prepare samples. They can also be used to quickly and adaptably execute sequencing analyses and to understand genomic epidemiology.

Item Type: Article (Journal)
Uncontrolled Keywords: SARS-COV-2; Oxford Nanopore
Subjects: R Medicine > RC Internal medicine > RC111 Infectious and Parasitic Diseases
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Internal Medicine
Depositing User: Asst. Dr. Ummu Afeera Zainulabid
Date Deposited: 05 Jul 2023 16:15
Last Modified: 05 Jul 2023 16:15
URI: http://irep.iium.edu.my/id/eprint/105346

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