Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source

Luthfi, Abdullah Amru Indera and Abdul, Peer Mohamed and Md Jahim, Jamaliah and Engliman, Nurul Sakinah and Jamali, Nur Syakina and Tan, Jian Ping and Abdul Manaf, Shareena Fairuz and Sajab, Mohd Shaiful and Bukhari, Nurul Adela (2023) Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source. Applied Sciences, 13 (1). pp. 1-18. E-ISSN 2076-3417

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Abstract

The effectiveness of biohydrogen conversion from biomass sources is governed by the selection of ideal biohydrogen-producing bacteria to achieve high and consistent production performance. The aim of this research was to isolate and identify a biohydrogen producer in local soil samples, as well as to evaluate its fermentability in biohydrogen production from oil palm empty fruit bunches (OPEFB). To this end, preliminary identification was performed using morphological, phenotype, biological, and 16s rRNA analyses. The fermentability of the isolate was further evaluated in a serum bottle and then in a 1.5 L anaerobic column bioreactor (ACBR) to investigate the potential for biohydrogen production using two OPEFB-based carbon sources: hydrolysate of ammonia fiber expansion (AFEX)-pretreated OPEFB and molasses from dilute acetic acid (DAA)-pretreated OPEFB. The isolated strain, Enterobacter sp. KBH 6958, was found to be capable of producing biohydrogen from various carbon sources via the pyruvate:ferredoxin oxidoreductase (PFOR) pathway. The cumulative conversion of AFEX OPEFB hydrolysate was 45% higher than that observed in DAA OPEFB molasses fermentation in the production of biohydrogen. The biohydrogen yield after fermenting AFEX OPEFB hydrolysate with Enterobacter sp. KBH 6958 was 1.55 mol H2/mol sugar, with a maximum productivity of 98.1 mL H2/h (4.01 mmol H2/L/h), whereas butyrate (10.6 mM), acetate (11.8 mM), and ethanol (4.56 mM) were found to be the major soluble metabolites. This study successfully demonstrated the biotechnological conversion of OPEFB into biohydrogen using a locally isolated strain, which not only solves environmental issues associated with the industry but may also offer a solution to the world’s energy insecurity.

Item Type:	Article (Journal)
Uncontrolled Keywords:	anaerobic column bioreactor; biohydrogen; characterization; Enterobacter KBH 6958; isolation; OPEFB
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Nurul Sakinah Engliman
Date Deposited:	07 Jun 2023 12:46
Last Modified:	08 Jun 2023 09:27
URI:	http://irep.iium.edu.my/id/eprint/104980

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