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Effectiveness of shrimp allergenic extract as an immunotherapy agent in mice model of gastrointestinal allergy

Sagitaras, Ilham Bagus and Dzikri Marhaeny, Honey and Pratama, Yusuf Alif and Ardianto, Chrismawan and Suasana, Dian and Nurhan, Ahmad Dzulfikri and Fatma Sari, Winda and Dinina, Fakhriyah and Bakhtiar, M. Taher and Khotib, Junaidi (2023) Effectiveness of shrimp allergenic extract as an immunotherapy agent in mice model of gastrointestinal allergy. Research Journal of Pharmacy and Technology, 16 (1). pp. 1-7. ISSN 0974-3618 E-ISSN 0974-360X

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Allergen extract as allergen-specific immunotherapy (AIT) is the only causative therapy and provides protection or tolerance to an allergen in the long term. However, allergen extracts from different countries may have different effectiveness. This study aimed to evaluate the effectiveness of Indonesian shrimp allergen extract (SAE) as an immunotherapy agent with a mouse model of allergies in the gastrointestinal tract. Mice were divided into five groups consisting of the naïve group, allergic group, and the allergic group received SAE immunotherapy at high dose (100µg/week), moderate dose (50µg/week), and low dose (10µg/week). Each group received treatment in the sensitization and desensitization phases, which was then followed by an oral challenge of SAE 100µg. The effectiveness of SAE immunotherapy was assessed based on the parameters of systemic allergic symptoms, IL-10 mRNA expression in ileum tissue, and IgG2a serum concentration. We found that SAE immunotherapy decreased the systemic allergic symptoms score, regardless of dosage, and the effect persisted on the third challenge. IgG2a as a parameter of humoral immunity showed a significant increase in the high-dose immunotherapy group, and IL-10mRNA expression as a parameter of cellular immunity also showed an increase in the high-dose group. Both data showed a dose-dependent manner. It can be concluded that SAE has excellent effectiveness as an immunotherapy agent and dose-dependent characteristics.

Item Type: Article (Journal)
Uncontrolled Keywords: Gastrointestinal allergy, Allergenic Shrimp Extract (ASE), Allergen specific-immunotherapy (AIT), IL-10, IgG2a, Neglected disease
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Muhammad Taher
Date Deposited: 17 Apr 2023 09:42
Last Modified: 17 Apr 2023 09:59
URI: http://irep.iium.edu.my/id/eprint/104429

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